Effects Of Coordination Mode Of 2-mercaptothiazoline On Reactivity Of Au(i) Compounds With Thiols And Sulfur-containing Proteins

dc.creatorAbbehausen
dc.creatorC.; Manzano
dc.creatorC. M.; Corbi
dc.creatorP. P.; Farrell
dc.creatorN. P.
dc.date2016
dc.datedez
dc.date2017-11-13T13:54:39Z
dc.date2017-11-13T13:54:39Z
dc.date.accessioned2018-03-29T06:08:06Z
dc.date.available2018-03-29T06:08:06Z
dc.identifierJournal Of Inorganic Biochemistry . Elsevier Science Inc , v. 165, p. 136 - 145, 2016.
dc.identifier0162-0134
dc.identifier1873-3344
dc.identifierWOS:000390615400016
dc.identifier10.1016/j.jinorgbio.2016.05.011
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0162013416301349?via%3Dihub
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/329461
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1366486
dc.descriptionGold(I) based drugs are interesting for their potential medical use. The relatively facile ligand substitution in linear gold(I) compounds makes the identification of active species complicated. Ligands such as PR3 and CN are likely to be carrier ligands due to their strong trans-directing properties and will dictate the nature of substitution reactions. The 2-mercaptothiazoline (mtz) ligand is an N,S-heterocyclic compound which presents an exocyclic thiol sulfur as well as a heterocyclic nitrogen. The coordination of mtz to transition metals can be modulated by the trans ligand and complexes with metal bound through the nitrogen and/or the exocyclic sulfur are known. Therefore, the complexes [NCAu(N-mtz)] (N-coordinated) and [(Ph3P)Au(S-mtz)] (S-coordinated) were investigated to compare the influence of CN- and PR3 as well as the coordination mode of the mtz ligand on reactivity with thiols and sulfur-containing proteins. As a further comparison the compound [(Ph3P)AuCN] was also studied. Human serum albumin, egg white lysozyme and, principally, the C-terminal zinc finger (ZF2) of the nucleocapsid NCp7protein of HIV-1 were studied. Results from zinc finger studies show that the coordination structure can determine the reactivity toward biomolecules. Due to ligand scrambling, the complex [NCAu(N-mtz)] forms very reactive species in solution generating [Nc(y)Au(x)-biomolecule] adducts, where x,y <= 3. The observation by mass spectrometry of [(CN)Au]-ZE confirms the ability of Au(l) compounds to form [(Ligand)Au] adducts on zinc fingers, in contrast to Au(III), where all ligands are lost upon reaction with the zinc finger. On the other hand, [(Ph3P)Au(S-mtz)] also generates the [(Ph3P)(2)Aur species due to ligand scrambling, that showed lower reactivity, probably due to steric hindrance. For this complex [(Ph3P)Au-biomolecule] and [Au-biomolecule] adducts are dominant The results corroborate the hypothesis of modulation through coordination as the reactivity clearly depends on not only the ligand, but also the coordination mode. (C) 2016 Elsevier Inc All rights reserved.
dc.description165
dc.description136
dc.description145
dc.description1st International Symposium on Clinical and Experimental Metallodrugs in Medicine - Cancer Chemotherapy (CEMM)
dc.descriptionDEC, 2015
dc.descriptionHonolulu, HI
dc.languageEnglish
dc.publisherElsevier Science INC
dc.publisherNew York
dc.relationJournal of Inorganic Biochemistry
dc.rightsfechado
dc.sourceWOS
dc.subjectGold(i)
dc.subjectSulfur Protein Interaction
dc.subjectZinc Finger
dc.subject2-mercaptothaizoline
dc.titleEffects Of Coordination Mode Of 2-mercaptothiazoline On Reactivity Of Au(i) Compounds With Thiols And Sulfur-containing Proteins
dc.typeActas de congresos


Este ítem pertenece a la siguiente institución