Design Of An Allele-specific Pcr Assay To Genotype The Rs12255372 Snp In A Pilot Study Of Association Between Common Tcf7l2 Polymorphisms And Type 2 Diabetes In Venezuelans

dc.creatorMoran
dc.creatorYeinmy; Labrador
dc.creatorLuis; Eugenia Camargo
dc.creatorMaria; Fernandez
dc.creatorDavid; Angel Chiurillo
dc.creatorMiguel
dc.date2016
dc.datejun
dc.date2017-11-13T13:45:41Z
dc.date2017-11-13T13:45:41Z
dc.date.accessioned2018-03-29T06:00:22Z
dc.date.available2018-03-29T06:00:22Z
dc.identifierArchives Of Endocrinology And Metabolism. Sbem-soc Brasil Endocrinologia & Metabologia, v. 60, p. 246 - 251, 2016.
dc.identifier2359-3997
dc.identifier2359-4292
dc.identifierWOS:000380358600010
dc.identifier10.1590/2359-3997000000064
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972016000300246&lng=en&tlng=en
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/329073
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1366098
dc.descriptionThe global burden of diabetes mellitus will impact strongly American countries in the coming decades. Type 2 diabetes mellitus (T2DM) is a multifactorial disease and the basis for its genetic susceptibility remains not fully understood. Different population studies have demonstrated that variants of the TCF7L2 gene are strongly associated with an increased risk of T2DM. Moreover, institutions or countries with limited budget to conduct genetic research need cost effective methods for detecting DNA variants. Subjects and methods: We standardized a rapid and simple allele-specific PCR method for genotyping the rs12255372 single nucleotide polymorphism (SNP) in a pilot study exploring the association of three TCF7L2 polymorphisms (rs7903146, rs12255372 and DG10S478) with T2DM in 70 patients and 73 controls from Venezuela. Results: The performance of the designed allele-specific PCR reaction for rs12255372 genotyping was reliable and accurate. Patients carrying the TCF7L2 rs7903146 T allele (CT + TT genotypes) and heterozygous CT genotype had a significantly higher risk for T2DM (OR = 2.9 and 2.3, respectively). Although rs12255372 and DG10S478 risk alleles predominated in T2DM group no statistical significance was found. Conclusions: We developed a novel allele-specific PCR method for easier and rapid detection of rs12255372 polymorphism without the use of expensive instrumentation and reagents. Our study in a relatively small sample of the Venezuelan population replicated the association of the rs7903146 SNP with T2DM. Further studies with larger sample size and more biochemical data should be conducted to explore the genetic basis of T2DM susceptibility in Venezuela.
dc.description60
dc.description3
dc.description246
dc.description251
dc.descriptionCDCHT-UCLA grant [001-CS-2013]
dc.languageEnglish
dc.publisherSBEM-Soc Brasil Endocrinologia & Metabologia
dc.publisherRio de Janeiro
dc.relationArchives of Endocrinology and Metabolism
dc.rightsaberto
dc.sourceWOS
dc.subjectType 2 Diabetes Mellitus
dc.subjectTcf7l2
dc.subjectAllele-specific Pcr
dc.subjectGene Polymorphism
dc.subjectVenezuela
dc.titleDesign Of An Allele-specific Pcr Assay To Genotype The Rs12255372 Snp In A Pilot Study Of Association Between Common Tcf7l2 Polymorphisms And Type 2 Diabetes In Venezuelans
dc.typeArtículos de revistas


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