Simultaneous Detection Of Lysine Metabolites By A Single Lc-ms/ms Method: Monitoring Lysine Degradation In Mouse Plasma

dc.creatorPena
dc.creatorIzabella A.; Marques
dc.creatorLygia A.; Laranjeira
dc.creatorAngelo B. A.; Yunes
dc.creatorJose A.; Eberlin
dc.creatorMarcos N.; Arruda
dc.creatorPaulo
dc.date2016
dc.datefev
dc.date2017-11-13T13:45:06Z
dc.date2017-11-13T13:45:06Z
dc.date.accessioned2018-03-29T05:59:44Z
dc.date.available2018-03-29T05:59:44Z
dc.identifierSpringerplus. Springer International Publishing Ag, v. 5, p. 172, 2016.
dc.identifier2193-1801
dc.identifierWOS:000371415200002
dc.identifier10.1186/s40064-016-1809-1
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/27026869
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328922
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365947
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionDetection and quantification of lysine degradation metabolites in plasma is necessary for the diagnosis and follow-up of diseases such as pyridoxine-dependent epilepsy. The principal metabolites involved in the disease are related to the first steps of lysine oxidation, either through the saccharopine or the pipecolate pathways. Currently, there are three different analytical methods used to assess the content of these metabolites in urine and plasma, but they require different sample preparations and analytical equipment. Here, we describe a protocol that calls for a simple sample preparation and uses liquid chromatography tandem mass spectrometry (LC-MS/MS) that allows simultaneous detection and quantification of underivatized L-saccharopine, L-aminoadipic acid, L-pipecolic acid, piperideine-6-carboxylate, L-glutamic acid, and pyridoxal-5-phosphate in plasma samples. To validate the method we analyzed the time course degradation after intraperitoneal injection of L-lysine in C57BL/6/J mice. We observed that the degradation of lysine through the saccharopine pathway reached a maximum within the first 2 h. At this time point there was an increase in the levels of the metabolites saccharopine, aminoadipic acid, and pipecolic acid by 3-, 24- and 3.4-fold, respectively, compared to time zero levels. These metabolites returned to basal levels after 4-6 h. In conclusion, we have developed a LC-MS/MS approach, which allows simultaneous analysis of lysine degradation metabolites without the need for derivatization.
dc.description5
dc.description172
dc.descriptionFAPESP [10/50114-4, 12/00235-5, 13/23920-8]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherSpringer International Publishing Ag
dc.publisherCham
dc.relationSpringerplus
dc.rightsaberto
dc.sourceWOS
dc.subjectLysine Catabolism
dc.subjectSaccharopine
dc.subjectAmino Acid
dc.subjectMass Spectrometry
dc.subjectPipecolic Acid
dc.titleSimultaneous Detection Of Lysine Metabolites By A Single Lc-ms/ms Method: Monitoring Lysine Degradation In Mouse Plasma
dc.typeArtículos de revistas


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