Low-level Laser Therapy (904 Nm) Counteracts Motor Deficit Of Mice Hind Limb Following Skeletal Muscle Injury Caused By Snakebite-mimicking Intramuscular Venom Injection

dc.creatorVieira
dc.creatorWillians Fernando; Kenzo-Kagawa
dc.creatorBruno; Cogo
dc.creatorJose Carlos; Baranauskas
dc.creatorVitor; da Cruz-Hofling
dc.creatorMaria Alice
dc.date2016
dc.datejul
dc.date2017-11-13T13:44:45Z
dc.date2017-11-13T13:44:45Z
dc.date.accessioned2018-03-29T05:59:26Z
dc.date.available2018-03-29T05:59:26Z
dc.identifierPlos One. Public Library Science, v. 11, p. , 2016.
dc.identifier1932-6203
dc.identifierWOS:000380005400179
dc.identifier10.1371/journal.pone.0158980
dc.identifierhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0158980
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328857
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365882
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionMyotoxins present in Bothrops venom disrupt the sarcolemma of muscle fibers leading to the release of sarcoplasmic proteins and loss of muscle homeostasis. Myonecrosis and tissue anoxia induced by vascularization impairment can lead to amputation or motor functional deficit. The objective of this study was to investigate the dynamic behavior of motor function in mice subjected to injection of Bothrops jararacussu venom (Bjssu) and exposed to low-level laser therapy (LLLT). Male Swiss mice received Bjssu injection (830 mu g/kg) into the medial portion of the right gastrocnemius muscle. Three hours later the injected region was irradiated with diode semiconductor Gallium Arsenide (GaAs-904 nm, 4 J/cm(2)) laser following by irradiation at 24, 48 and 72 hours. Saline injection (0.9% NaCl) was used as control. Gait analysis was performed 24 hours before Bjssu injection and at every period post-Bjssu using CatWalk method. Data from spatiotemporal parameters Stand, Maximum Intensity, Swing, Swing Speed, Stride Length and Step Cycle were considered. The period of 3 hours post venom-induced injury was considered critical for all parameters evaluated in the right hindlimb. Differences (p<0.05) were concentrated in venom and venom + placebo laser groups during the 3 hours post-injury period, in which the values of stand of most animals were null. After this period, the gait characteristics were re-established for all parameters. The venom + laser group kept the values at 3 hours post-Bjssu equal to that at 24 hours before Bjssu injection indicating that the GaAs laser therapy improved spatially and temporally gait parameters at the critical injury period caused by Bjssu. This is the first study to analyze with cutting edge technology the gait functional deficits caused by snake envenoming and gait gains produced by GaAs laser irradiation. In this sense, the study fills a gap on the field of motor function after laser treatment following snake envenoming.
dc.description11
dc.description7
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [05/53625-1]
dc.descriptionConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [488792/2011, 486142/2012-4]
dc.descriptionCNPq [305099/2011-6]
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) at the Departamento de Semicondutores, Instrumentos e Fotonica (FEEC-UNICAMP)
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) at Departamento de Bioquimica e Biologia Tecidual (IB-UNICAMP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageEnglish
dc.publisherPublic Library Science
dc.publisherSan Francisco
dc.relationPlos One
dc.rightsaberto
dc.sourceWOS
dc.titleLow-level Laser Therapy (904 Nm) Counteracts Motor Deficit Of Mice Hind Limb Following Skeletal Muscle Injury Caused By Snakebite-mimicking Intramuscular Venom Injection
dc.typeArtículos de revistas


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