Long-standing Motor And Sensory Recovery Following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair

dc.creatorBiscola
dc.creatorNatalia Perussi; Cartarozzi
dc.creatorLuciana Politti; Ferreira Junior
dc.creatorRui Seabra; Barraviera
dc.creatorBenedito; Rodrigues de Oliveira
dc.creatorAlexandre Leite
dc.date2016
dc.date2017-11-13T13:44:32Z
dc.date2017-11-13T13:44:32Z
dc.date.accessioned2018-03-29T05:59:11Z
dc.date.available2018-03-29T05:59:11Z
dc.identifierNeural Plasticity. Hindawi Publishing Corp, p. , 2016.
dc.identifier2090-5904
dc.identifier1687-5443
dc.identifierWOS:000379469200001
dc.identifier10.1155/2016/9028126
dc.identifierhttps://www.hindawi.com/journals/np/2016/9028126/
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328792
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365817
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionBrachial plexus lesion results in loss of motor and sensory function, being more harmful in the neonate. Therefore, this study evaluated neuroprotection and regeneration after neonatal peripheral nerve coaptation with fibrin sealant. Thus, P2 neonatal Lewis rats were divided into three groups: AX: sciatic nerve axotomy (SNA) without treatment; AX+FS: SNA followed by end-to-end coaptation with fibrin sealant derived from snake venom; AX+CFS: SNA followed by end-to-end coaptation with commercial fibrin sealant. Results were analyzed 4, 8, and 12 weeks after lesion. Astrogliosis, microglial reaction, and synapse preservation were evaluated by immunohistochemistry. Neuronal survival, axonal regeneration, and ultrastructural changes at ventral spinal cord were also investigated. Sensory-motor recovery was behaviorally studied. Coaptation preserved synaptic covering on lesioned motoneurons and led to neuronal survival. Reactive gliosis and microglial reaction decreased in the same groups (AX+FS, AX+CFS) at 4 weeks. Regarding axonal regeneration, coaptation allowed recovery of greater number of myelinated fibers, with improved morphometric parameters. Preservation of inhibitory synaptic terminals was accompanied by significant improvement in the motor as well as in the nociceptive recovery. Overall, the present data suggest that acute repair of neonatal peripheral nerves with fibrin sealant results in neuroprotection and regeneration of motor and sensory axons.
dc.descriptionSao Paulo Research Foundation (FAPESP) [2014/06892-3, 2012/19646-6, 2012/08101-8, 2011/23236-4, 2009/53846-9]
dc.descriptionFAPESP [2011/23377-7]
dc.descriptionCNPq (Brazil) [300552/2013-9, 310207/2011-8]
dc.descriptionCAPES [23038.006285/2011-21]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageEnglish
dc.publisherHindawi Publishing Corp
dc.publisherNew York
dc.relationNeural Plasticity
dc.rightsaberto
dc.sourceWOS
dc.subjectMesenchymal Stem-cells
dc.subjectDurissus-terrificus Venom
dc.subjectWalking Track Analysis
dc.subjectSpinal-cord-injury
dc.subjectSnake-venom
dc.subjectAxotomized Motoneurons
dc.subjectSynaptic Plasticity
dc.subjectAxonal Regeneration
dc.subjectFunctional Recovery
dc.subjectGlial Reaction
dc.titleLong-standing Motor And Sensory Recovery Following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair
dc.typeArtículos de revistas


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