An In Vitro Digestion Method Adapted For Carotenoids And Carotenoid Esters: Moving Forward Towards Standardization

dc.creatorRodrigues
dc.creatorDaniele Bobrowski; Barros Mariutti
dc.creatorLilian Regina; Mercadante
dc.creatorAdriana Zerlotti
dc.date2016
dc.date2017-11-13T13:44:00Z
dc.date2017-11-13T13:44:00Z
dc.date.accessioned2018-03-29T05:58:43Z
dc.date.available2018-03-29T05:58:43Z
dc.identifierFood & Function. Royal Soc Chemistry, v. 7, p. 4992 - 5001, 2016.
dc.identifier2042-6496
dc.identifier2042-650X
dc.identifierWOS:000395375900027
dc.identifier10.1039/c6fo01293k
dc.identifierhttp://pubs.rsc.org/en/Content/ArticleLanding/2016/FO/C6FO01293K#!divAbstract
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328682
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365707
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionIn vitro digestion methods are a useful approach to predict the bioaccessibility of food components and overcome some limitations or disadvantages associated with in vivo methodologies. Recently, the INFOGEST network published a static method of in vitro digestion with a proposal for assay standardization. The INFOGEST method is not specific for any food component; therefore, we aimed to adapt this method to assess the in vitro bioaccessibility of carotenoids and carotenoid esters in a model fruit (Byrsonima crassifolia). Two additional steps were coupled to the in vitro digestion procedure, centrifugation at 20 000g for the separation of the aqueous phase containing mixed micelles and exhaustive carotenoid extraction with an organic solvent. The effect of electrolytes, enzymes and bile acids on carotenoid micellarization and stability was also tested. The results were compared with those found with a simpler method that has already been used for carotenoid bioaccessibility analysis. These values were in the expected range for free carotenoids (5-29%), monoesters (9-26%) and diesters (4-28%). In general, the in vitro bioaccessibility of carotenoids assessed by the adapted INFOGEST method was significantly higher (p < 0.05) than those assessed by the simplest protocol, with or without the addition of simulated fluids. Although no trend was observed, differences in bioaccessibility values depended on the carotenoid form (free, monoester or diester), isomerization (Z/E) and the in vitro digestion protocol. To the best of our knowledge, it was the first time that a systematic identification of carotenoid esters by HPLC-DAD-MS/MS after in vitro digestion using the INFOGEST protocol was carried out.
dc.description7
dc.description12
dc.description4992
dc.description5001
dc.descriptionFAPESP [2012/20988-8, 2013/23218-1, 2012/01506-2]
dc.descriptionCNPq [308484/2014-2]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherRoyal Soc Chemistry
dc.publisherCambridge
dc.relationFood & Function
dc.rightsfechado
dc.sourceWOS
dc.subjectHuman Intestinal-cells
dc.subjectLutein Bioavailability
dc.subjectLiquid-chromatography
dc.subjectDivalent Minerals
dc.subjectReactive Oxygen
dc.subjectLycium-barbarum
dc.subjectLipid Droplets
dc.subjectFish Oils
dc.subjectBioaccessibility
dc.subjectDietary
dc.titleAn In Vitro Digestion Method Adapted For Carotenoids And Carotenoid Esters: Moving Forward Towards Standardization
dc.typeArtículos de revistas


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