Root Growth Restraint Can Be An Acclimatory Response To Low Ph And Is Associated With Reduced Cell Mortality: A Possible Role Of Class Iii Peroxidases And Nadph Oxidases

dc.creatorGracas
dc.creatorJ. P.; Ruiz-Romero
dc.creatorR.; Figueiredo
dc.creatorL. D.; Mattiello
dc.creatorL.; Peres
dc.creatorL. E. P.; Vitorello
dc.creatorV. A.
dc.date2016
dc.datejul
dc.date2017-11-13T13:26:02Z
dc.date2017-11-13T13:26:02Z
dc.date.accessioned2018-03-29T05:57:56Z
dc.date.available2018-03-29T05:57:56Z
dc.identifierPlant Biology. Wiley-blackwell, v. 18, p. 658 - 668, 2016.
dc.identifier1435-8603
dc.identifier1438-8677
dc.identifierWOS:000378628700014
dc.identifier10.1111/plb.12443
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/plb.12443/abstract
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328503
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365528
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionLow pH (<5.0) can significantly decrease root growth but whether this is a direct effect of H+ or an active plant response is examined here. Tomato (Solanum lycopersicum cv Micro-Tom) roots were exposed directly or gradually to low pH through step-wise changes in pH over periods ranging from 4 to 24h. Roots exposed gradually to pH 4.5 grew even less than those exposed directly, indicating a plant-coordinated response. Direct exposure to pH 4.0 suppressed root growth and caused high cell mortality, in contrast to roots exposed gradually, in which growth remained inhibited but cell viability was maintained. Total class III peroxidase activity increased significantly in all low pH treatments, but was not correlated with the observed differential responses. Use of the enzyme inhibitors salicylhydroxamic acid (SHAM) or diphenyleneiodonium chloride (DPI) suggest that peroxidase and, to a lesser extent, NADPH oxidase were required to prevent or reduce injury in all low pH treatments. However, a role for other enzymes, such as the alternative oxidase is also possible. The results with SHAM, but not DPI, were confirmed in tobacco BY-2 cells. Our results indicate that root growth inhibition from low pH can be part of an active plant response, and suggest that peroxidases may have a critical early role in reducing loss of cell viability and in the observed root growth constraint.
dc.description18
dc.description4
dc.description658
dc.description668
dc.descriptionCAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
dc.descriptionFAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
dc.descriptionCENIPALMA (Corporacion Centro de Investigacion en Palma de Aceite)
dc.descriptionFAPESP [2009/53998-3]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherWiley-Blackwell
dc.publisherHoboken
dc.relationPlant Biology
dc.rightsfechado
dc.sourceWOS
dc.subjectCell Viability
dc.subjectCell Wall
dc.subjectClass Iii Peroxidase
dc.subjectLow Ph
dc.subjectNadph Oxidase
dc.subjectRoot Growth
dc.titleRoot Growth Restraint Can Be An Acclimatory Response To Low Ph And Is Associated With Reduced Cell Mortality: A Possible Role Of Class Iii Peroxidases And Nadph Oxidases
dc.typeArtículos de revistas


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