Biochemical Characterization Of Solvent, Salt, Surfactant And Oxidizing Agent Tolerant Proteases From Aspergillus Niger Produced In Different Agroindustrial Wastes

dc.creatorSoares de Castro
dc.creatorRuann Janser; Soares
dc.creatorMarilia Herculano; Mangabeira Albernaz
dc.creatorJuliana Reolon; Sato
dc.creatorHelia Harumi
dc.date2016
dc.datejan
dc.date2017-11-13T13:25:19Z
dc.date2017-11-13T13:25:19Z
dc.date.accessioned2018-03-29T05:57:43Z
dc.date.available2018-03-29T05:57:43Z
dc.identifierBiocatalysis And Agricultural Biotechnology. Elsevier Science Bv, v. 5, p. 94 - 98, 2016.
dc.identifier1878-8181
dc.identifierWOS:000371892900013
dc.identifier10.1016/j.bcab.2015.12.011
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S1878818115300311
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328480
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365505
dc.descriptionProteases produced by Aspergillus niger LBA 02 under solid state fermentation using different agroindustrial wastes had their enzyme activities tested in the presence of organic solvents, NaCI, surfactants and oxidizing agents. The results showed that according to the fermentation substrate, the microorganism was able to secrete proteases with different biochemical characteristics. When wheat bran was used as the substrate, the proteases showed the highest relative activity in 50% (v/v) petroleum ether, reaching 114.3%, while the proteases produced in soybean meal and cottonseed meal presented 94.7% and 106.1%, respectively. These enzymes also presented high relative activity values in 50% (v/v) chloroform, retaining up to 85% of their initial protease activities after 3 h at 37 degrees C. When surfactants and oxidizing agents were tested, the proteases produced in soybean meal proved to have remarkable and the highest activity followed by the proteases produced in wheat bran and cottonseed meal. Since the protease produced in wheat bran showed considerable tolerance at high concentrations of NaCI, with relative activity of 61.1% at 15% NaCI (w/v), while the proteases produced in soybean meal and cottonseed meal retained 48.7% and 46.3% of their initial activities, respectively. This work provides an interesting approach about the modulation of biochemical properties of enzymes in response to different substrates, what can be used for obtaining proteases with specific applications. (C) 2015 Elsevier Ltd. All rights reserved.
dc.description5
dc.description94
dc.description98
dc.descriptionDepartment of Food Science, School of Food Engineering, University of Campinas
dc.languageEnglish
dc.publisherElsevier Science BV
dc.publisherAmsterdam
dc.relationBiocatalysis and Agricultural Biotechnology
dc.rightsfechado
dc.sourceWOS
dc.subjectProtease
dc.subjectBiochemical Properties
dc.subjectAgroindustrial Wastes
dc.subjectChemical Agents
dc.subjectSalt-tolerant
dc.titleBiochemical Characterization Of Solvent, Salt, Surfactant And Oxidizing Agent Tolerant Proteases From Aspergillus Niger Produced In Different Agroindustrial Wastes
dc.typeArtículos de revistas


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