Carcinoma Ex-pleomorphic Adenoma Derived From Recurrent Pleomorphic Adenoma Shows Important Difference By Array Cgh Compared To Recurrent Pleomorphic Adenoma Without Malignant Transformation

dc.creatorMariano
dc.creatorFernanda Viviane; Giovanetti
dc.creatorKarina; Vidal Saccomani
dc.creatorLuis Fernando; Del Negro
dc.creatorAndre; Kowalski
dc.creatorLuiz Paulo; Victorino Krepischi
dc.creatorAna Cristina; Altemani
dc.creatorAlbina
dc.date2016
dc.datenov-dez
dc.date2017-11-13T13:24:57Z
dc.date2017-11-13T13:24:57Z
dc.date.accessioned2018-03-29T05:57:25Z
dc.date.available2018-03-29T05:57:25Z
dc.identifierBrazilian Journal Of Otorhinolaryngology. Assoc Brasileira Otorrinolaringologia & Cirurgia Cervicofacial, v. 82, p. 687 - 694, 2016.
dc.identifier1808-8694
dc.identifier1808-8686
dc.identifierWOS:000389967300012
dc.identifier10.1016/j.bjorl.2015.12.004
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S1808869416000318?via%3Dihub
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328409
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365434
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionA key step of cancer development is the progressive accumulation of genomic changes resulting in disruption of several biological mechanisms. Carcinoma ex-pleomorphic adenoma (CXPA) is an aggressive neoplasm that arises from a pleomorphic adenoma. CXPA derived from a recurrent PA (RPA) has been rarely reported, and the genomic changes associated with these tumors have not yet been studied. Objective: We analyzed CXPA from RPAs and RPAs without malignant transformation using array comparative genomic hybridization (array-CGH) to identify somatic copy number alterations and affected genes. Methods: DNA samples extracted from FFPE tumors were submitted to array-CGH investigation, and data was analyzed by Nexus Copy Number Discovery Edition v.7. Results: No somatic copy number alterations were found in RPAs without malignant transformation. As for CXPA from RPA, although genomic profiles were unique for each case, we detected some chromosomal regions that appear to be preferentially affected by copy number alterations. The first case of CXPA-RPA (frankly invasive myoepithelial carcinoma) showed copy number alterations affecting 1p36.33p13, 5p and chromosomes 3 and 8. The second case of CXPA-RPA (frankly invasive epithelial-myoepithelial carcinoma) showed several alterations at chromosomes 3, 8, and 16, with two amplifications at 8p12p11.21 and 12q14.3q21.2. The third case of CXPA-RPA (minimally invasive epithelial-myoepithelial carcinoma) exhibited amplifications at 12q13.3q14.1, 12q14.3, and 12q15. Conclusion: The occurrence of gains at chromosomes 3 and 8 and genomic amplifications at 8p and 12q, mainly those encompassing the HMGA2, MDM2, WIF1, WHSC1L1, LIRG3, CDK4 in CXAP from RPA can be a significant promotional factor in malignant transformation. (C) 2016 Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervico-Facial. Published by Elsevier Editora Ltda.
dc.description82
dc.description6
dc.description687
dc.description694
dc.descriptionFAPESP [2011/23204-5, 2011/23366-5]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherAssoc Brasileira Otorrinolaringologia & Cirurgia Cervicofacial
dc.publisherSão Paulo
dc.relationBrazilian Journal of Otorhinolaryngology
dc.rightsaberto
dc.sourceWOS
dc.subjectCarcinoma Ex-pleomorphic Adenoma
dc.subjectRecurrent Pleomorphic Adenoma
dc.subjectSomatic Copy Number Alterations
dc.subjectAcgh
dc.titleCarcinoma Ex-pleomorphic Adenoma Derived From Recurrent Pleomorphic Adenoma Shows Important Difference By Array Cgh Compared To Recurrent Pleomorphic Adenoma Without Malignant Transformation
dc.typeArtículos de revistas


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