Enamel And Dentine Demineralization By A Combination Of Starch And Sucrose In A Biofilm - Caries Model

dc.creatorBotelho
dc.creatorJuliana Nunes; Villegas-Salinas
dc.creatorMario; Troncoso-Gajardo
dc.creatorPia; Giacaman
dc.creatorRodrigo Andres; Cury
dc.creatorJaime Aparecido
dc.date2016
dc.date2017-11-13T13:24:49Z
dc.date2017-11-13T13:24:49Z
dc.date.accessioned2018-03-29T05:57:18Z
dc.date.available2018-03-29T05:57:18Z
dc.identifierBrazilian Oral Research. Sociedade Brasileira De Pesquisa Odontologica, v. 30, p. , 2016.
dc.identifier1806-8324
dc.identifier1678-8079
dc.identifierWOS:000378324000046
dc.identifier10.1590/1807-3107BOR-2016.vol30.0052
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100250&lng=en&tlng=en
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328379
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365404
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionSucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine. However, the cariogenicity of the combination of starch and sucrose remains unclear. The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization. Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva. They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose). To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium. Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified. Dentine and enamel demineralization was assessed by determining the percentage of surface hardness loss. Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone. The findings suggest that starch increases the cariogenic potential of sucrose.
dc.description30
dc.description1
dc.descriptionConselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq [475178/2011-4, 305310/2011-9]
dc.descriptionFundacao de Desenvolvimento da Unicamp - FUNCAMP [65/91, 4252]
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherSociedade Brasileira de Pesquisa Odontologica
dc.publisherSão Paulo
dc.relationBrazilian Oral Research
dc.rightsaberto
dc.sourceWOS
dc.subjectAmylases
dc.subjectBiofilms
dc.subjectDental Caries
dc.subjectDietary Carbohydrates
dc.subjectTooth Demineralization
dc.titleEnamel And Dentine Demineralization By A Combination Of Starch And Sucrose In A Biofilm - Caries Model
dc.typeArtículos de revistas


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