dc.creatorValadares
dc.creatorFernanda; Goncalves
dc.creatorThiago A.; Goncalves
dc.creatorDayelle S. P. O.; Segato
dc.creatorFernando; Romanel
dc.creatorElisson; Milagres
dc.creatorAdriane M. F.; Squina
dc.creatorFabio M.; Ferraz
dc.creatorAndre
dc.date2016
dc.datemaio
dc.date2017-11-13T13:24:34Z
dc.date2017-11-13T13:24:34Z
dc.date.accessioned2018-03-29T05:57:05Z
dc.date.available2018-03-29T05:57:05Z
dc.identifierBiotechnology For Biofuels. Biomed Central Ltd, v. 9, p. , 2016.
dc.identifier1754-6834
dc.identifierWOS:000377258800002
dc.identifier10.1186/s13068-016-0525-y
dc.identifierhttps://biotechnologyforbiofuels.biomedcentral.com.ez88.periodicos.capes.gov.br/articles/10.1186/s13068-016-0525-y
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328328
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365353
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionGlycoside hydrolases (GHs) and accessory proteins are key components for efficient and cost-effective enzymatic hydrolysis of polysaccharides in modern, biochemically based biorefineries. Currently, commercialized GHs and accessory proteins are produced by ascomycetes. However, the role of wood decay basidiomycetes proteins in biomass saccharification has not been extensively pursued. Wood decay fungi degrade polysaccharides in highly lignified tissues in natural environments, and are a promising enzyme source for improving enzymatic cocktails that are designed for in vitro lignocellulose conversion. Results: GHs and accessory proteins were produced by representative brown-and white-rot fungi, Laetiporus sulphureus and Pleurotus ostreatus, respectively. Concentrated protein extracts were then used to amend commercial enzymatic cocktails for saccharification of alkaline-sulfite pretreated sugarcane bagasse. The main enzymatic activities found in the wood decay fungal protein extracts were attributed to endoglucanases, xylanases and beta-glucosidases. Cellobiohydrolase (CBH) activities in the L. sulphureus and P. ostreatus extracts were low and nonexistent, respectively. The initial glucan conversion rates were boosted when the wood decay fungal proteins were used to replace half of the enzymes from the commercial cocktails. L. sulphureus proteins increased the glucan conversion levels, with values above those observed for the full load of commercial enzymes. Wood decay fungal proteins also enhanced the xylan conversion efficiency due to their high xylanase activities. Proteomic studies revealed 104 and 45 different proteins in the P. ostreatus and L. sulphureus extracts, respectively. The enhancement of the saccharification of alkaline-pretreated substrates by the modified enzymatic cocktails was attributed to the following protein families: GH5- and GH45-endoglucanases, GH3-beta-glucosidases, and GH10-xylanases. Conclusions: The extracellular proteins produced by wood decay fungi provide useful tools to improve commercial enzyme cocktails that are currently used for the saccharification of alkaline-pretreated lignocellulosic substrates. The relevant proteins encompass multiple glycoside hydrolase families, including the GH5-and GH45-endoglucanases, GH3-beta-glucosidases, and GH10-xylanases.
dc.description9
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [08/56256-5, 2014/06923-6]
dc.descriptionConselho Nacional de Pesquisa (CNPq) [442333/2014-5, 310186/2014-5, 140796/2013-4]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherBiomed Central Ltd
dc.publisherLondon
dc.relationBiotechnology for Biofuels
dc.rightsfechado
dc.sourceWOS
dc.subjectAlkaline Pretreatment
dc.subjectBiorefinery
dc.subjectGlycoside-hydrolases
dc.subjectEnzyme Cocktails
dc.subjectSugarcane
dc.subjectWood Decay Fungi
dc.titleExploring Glycoside Hydrolases And Accessory Proteins From Wood Decay Fungi To Enhance Sugarcane Bagasse Saccharification
dc.typeArtículos de revistas


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