The Mqsra Toxin-antitoxin System From Xylella Fastidiosa Plays A Key Role In Bacterial Fitness, Pathogenicity, And Persister Cell Formation

dc.creatorMerfa
dc.creatorMarcus V.; Niza
dc.creatorBarbara; Takita
dc.creatorMarco A.; De Souza
dc.creatorAlessandra A.
dc.date2016
dc.datejun
dc.date2017-11-13T13:23:32Z
dc.date2017-11-13T13:23:32Z
dc.date.accessioned2018-03-29T05:56:10Z
dc.date.available2018-03-29T05:56:10Z
dc.identifierFrontiers In Microbiology. Frontiers Media Sa, v. 7, p. , 2016.
dc.identifier1664-302X
dc.identifierWOS:000377597900001
dc.identifier10.3389/fmicb.2016.00904
dc.identifierhttp://journal.frontiersin.org/article/10.3389/fmicb.2016.00904/full
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328120
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365145
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThrough the formation of persister cells, bacteria exhibit tolerance to multidrug and other environmental stresses without undergoing genetic changes. The toxin-antitoxin (TA) systems are involved in the formation of persister cells because they are able to induce cell dormancy. Among the TA systems, the MqsRA system has been observed to be highly induced in persister cells of Xylella fastidiosa (causal agent of citrus variegated chlorosis-CVC) activated by copper stress, and has been described in Escherichia coil as related to the formation of persister cells and biofilms. Thus, we evaluated the role of this TA system in X. fastidiosa by overexpressing the MqsR toxin, and verified that the toxin positively regulated biofilm formation and negatively cell movement, resulting in reduced pathogenicity in citrus plants. The overexpression of MqsR also increased the formation of persister cells under copper stress. Analysis of the gene and protein expression showed that this system likely has an autoregulation mechanism to express the toxin and antitoxin in the most beneficial ratio for the cell to oppose stress. Our results suggest that this TA system plays a key role in the adaptation and survival of X fastidiosa and reveal new insights into the physiology of phytopathogen host interactions.
dc.description7
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo [2010/50712-9, 2013/17485-7]
dc.descriptionCNPq
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherFrontiers Media SA
dc.publisherLausanne
dc.relationFrontiers in Microbiology
dc.rightsaberto
dc.sourceWOS
dc.subjectPlant Pathogen
dc.subjectBiofilm
dc.subjectToxin-antitoxin System
dc.subjectPersister Cell
dc.subjectCopper Stress
dc.titleThe Mqsra Toxin-antitoxin System From Xylella Fastidiosa Plays A Key Role In Bacterial Fitness, Pathogenicity, And Persister Cell Formation
dc.typeArtículos de revistas


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