Brasil | Artículos de revistas

Subtelomeric I-scel-mediated Double-strand Breaks Are Repaired By Homologous Recombination In Trypanosoma Cruzi

dc.creatorChiurillo
dc.creatorMiguel A.; Moraes Barros
dc.creatorRoberto R.; Souza
dc.creatorRenata T.; Marini
dc.creatorMarjorie M.; Antonio
dc.creatorCristiane R.; Cortez
dc.creatorDanielle R.; Curto
dc.creatorMaria A.; Lorenz
dc.creatorHernan A.; Schijman
dc.creatorAlejandro G.; Ramirez
dc.creatorJose L.; da Silveira
dc.creatorJose F.
dc.date2016
dc.datedez
dc.date2017-11-13T13:23:31Z
dc.date2017-11-13T13:23:31Z
dc.date.accessioned2018-03-29T05:56:09Z
dc.date.available2018-03-29T05:56:09Z
dc.identifierFrontiers In Microbiology. Frontiers Media Sa, v. 7, p. , 2016.
dc.identifier1664-302X
dc.identifierWOS:000390654600001
dc.identifier10.3389/fmicb.2016.02041
dc.identifierhttp://journal.frontiersin.org/article/10.3389/fmicb.2016.02041/full
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328118
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365143
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionTrypanosoma cruzi chromosome ends are enriched in surface protein genes and pseudogenes (e.g., trans-sialidases) surrounded by repetitive sequences. It has been proposed that the extensive sequence variability among members of these protein families could play a role in parasite infectivity and evasion of host immune response. In previous reports we showed evidence suggesting that sequences located in these regions are subjected to recombination. To support this hypothesis we introduced a double-strand break (DSB) at a specific target site in a I cruzi subtelomeric region cloned into an artificial chromosome (pTAC). This construct was used to transfect T. cruzi epimastigotes expressing the I-Scel meganuclease. Examination of the repaired sequences showed that DNA repair occurred only through homologous recombination (HR) with endogenous subtelomeric sequences. Our findings suggest that DSBs in subtelomeric repetitive sequences followed by HR between them may contribute to increased variability in T. cruzi multigene families.
dc.description7
dc.descriptionFAPESP [11/51693-0, 11/51475-3]
dc.descriptionCNPq [306591/2015-4]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherFrontiers Media SA
dc.publisherLausanne
dc.relationFrontiers in Microbiology
dc.rightsaberto
dc.sourceWOS
dc.subjectT. Cruzi
dc.subjectTelomere
dc.subjectI-scel Meganuclease
dc.subjectDouble-strand Break
dc.subjectHomologous Recombination
dc.subjectDna Repair
dc.subjectArtificial Chromosomes
dc.titleSubtelomeric I-scel-mediated Double-strand Breaks Are Repaired By Homologous Recombination In Trypanosoma Cruzi
dc.typeArtículos de revistas


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