Fluorescent Lifetime Imaging Microscopy Using Europium Complexes Improves Atherosclerotic Plaques Discrimination

dc.creatorSicchieri
dc.creatorLeticia Bonfante; Natal
dc.creatorRodrigo de Andrade; Courrol
dc.creatorLilia Coronato
dc.date2016
dc.dateout
dc.date2017-11-13T13:22:58Z
dc.date2017-11-13T13:22:58Z
dc.date.accessioned2018-03-29T05:55:41Z
dc.date.available2018-03-29T05:55:41Z
dc.identifierInternational Journal Of Cardiovascular. Springer, v. 32, p. 1595 - 1604, 2016.
dc.identifier1569-5794
dc.identifier1573-0743
dc.identifierWOS:000383831300016
dc.identifier10.1007/s10554-016-0936-7
dc.identifierhttps://link-springer-com.ez88.periodicos.capes.gov.br/article/10.1007%2Fs10554-016-0936-7
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365031
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionThe objective of this study is to characterize arterial tissue with and without atherosclerosis by fluorescence lifetime imaging microscopy (FLIM) using Europium Chlortetracycline complex (EuCTc) as fluorescent marker. For this study, twelve rabbits were randomly divided into a control group (CG) and an experimental group (EG), where they were fed a normal and hypercholesterolemic diet, respectively, and were treated for 60 days. Cryosections of the aortic arch specimens were cut in a vertical plane, mounted on glass slides, and stained with Europium (Eu), Chlortetracycline (CTc), Europium Chlortetracycline (EuCTc), and Europium Chlortetracycline Magnesium (EuCTcMg) solutions. FLIM images were obtained with excitation at 405 nm. The average autofluorescence lifetime within plaque depositions was similar to 1.36 ns. Reduced plaque autofluorescence lifetimes of 0.23 and 0.31 ns were observed on incubation with EuCTc and EuCTcMg respectively. It was observed a quenching of collagen, cholesterol and TG emission spectra increasing EuCTc concentration. The drastic reduction in fluorescence lifetimes is due to a resonant energy transfer between collagen, triglycerides, cholesterol and europium complexes, quenching fluorescence.
dc.description32
dc.description10
dc.description1595
dc.description1604
dc.descriptionFapesp [2014/06960-9]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherSpringer
dc.publisherDordrecht
dc.relationInternational Journal of Cardiovascular
dc.rightsfechado
dc.sourceWOS
dc.subjectAtherosclerosis
dc.subjectFlim
dc.subjectEuropium Chlortetracycline
dc.subjectFret
dc.subjectCollagen
dc.subjectCholesterol
dc.subjectTriglycerides
dc.titleFluorescent Lifetime Imaging Microscopy Using Europium Complexes Improves Atherosclerotic Plaques Discrimination
dc.typeArtículos de revistas


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