Chrysobalanus Icaco L. Fruits Inhibit Nadph Oxidase Complex And Protect Dna Against Doxorubicin-induced Damage In Wistar Male Rats

dc.creatorVenancio
dc.creatorVinicius Paula; Marques
dc.creatorMarcella Camargo; Almeida
dc.creatorMara Ribeiro; Barros Mariutti
dc.creatorLilian Regina; de Oliveira Souza
dc.creatorVanessa Cristina; Barbosa
dc.creatorFernando
dc.creatorJr.; Pires Bianchi
dc.creatorMaria Lourdes; Marzocchi-Machado
dc.creatorCleni Mara; Mercadante
dc.creatorAdriana Zerlotti; Greggi Antunes
dc.creatorLusania Maria
dc.date2016
dc.date2017-11-13T13:22:19Z
dc.date2017-11-13T13:22:19Z
dc.date.accessioned2018-03-29T05:55:06Z
dc.date.available2018-03-29T05:55:06Z
dc.identifierJournal Of Toxicology And Environmental Health-part A-current Issues. Taylor & Francis Inc, v. 79, p. 885 - 893, 2016.
dc.identifier1528-7394
dc.identifier1087-2620
dc.identifierWOS:000384551300001
dc.identifier10.1080/15287394.2016.1193454
dc.identifierhttp://online.liebertpub.com/doi/abs/10.1089/jmf.2015.0034
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327862
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1364887
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionChrysobalanus icaco L. is an underexplored plant found in tropical areas around the globe. Currently, there is no apparent information regarding the effects C. icaco fruits may exert in vivo or potential role in health promotion. This study aimed at providing evidence regarding the in vivo influence of this fruit on antigenotoxicity, antimutagenicity, and oxidative stress in rats. Male Wistar rats were treated with 100, 200, or 400 mg/kg body weight (bw)/d C. icaco fruit for 14 d. Doxorubicin (DXR, 15 mg/kg bw, ip) was used for DNA damaging and as an oxidant to generate reactive oxygen species (ROS). Genomic instability was assessed by the comet assay and micronucleus (MN) test, while antioxidant activity was determined by oxidative burst of neutrophils. Chrysobalanus icaco fruit polyphenols were quantified and characterized by high-performance liquid chromatography coupled to a diode array detector and tandem mass spectrometer (HPLC-DAD-MS/MS). The concentrations of 19 chemical elements were determined by inductively coupled plasma-mass spectroscopy (ICP-MS). Significant amounts of polyphenols, magnesium, and selenium were found in C. icaco fruit. This fruit displayed in vivo antioxidant activity against DXR-induced damage in rat peripheral blood neutrophils, antigenotoxicity in peripheral blood cells, and antimutagenicity in bone-marrow cells and peripheral blood cells. Correlation analyses between endpoints examined indicated that the mechanism underlying chemopreventive actions of C. icaco fruit was attributed to inhibition of NADPH oxidase complex manifested as low levels of DNA damage in animals exposed to DXR. Data indicate that phytochemicals and minerals in C. icaco fruit protect DNA against damage in vivo associated with their antioxidant properties.
dc.description79
dc.description20
dc.description885
dc.description893
dc.descriptionSao Paulo Research Foundation (FAPESP) [2011/21471-6]
dc.descriptionNational Council for Scientific and Technological Development (CNPq) [471700/2012-6]
dc.descriptionCoordination for the Improvement of Higher Education Personnel (CAPES)
dc.descriptionCNPq
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherTaylor & Francis Inc
dc.publisherPhiladelphia
dc.relationJournal of Toxicology and Environmental Health-Part A-Current Issues
dc.rightsfechado
dc.sourceWOS
dc.titleChrysobalanus Icaco L. Fruits Inhibit Nadph Oxidase Complex And Protect Dna Against Doxorubicin-induced Damage In Wistar Male Rats
dc.typeArtículos de revistas


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