A Novel Approach For The Production And Purification Of Mannosylerythritol Lipids (mel) By Pseudozyma Tsukubaensis Using Cassava Wastewater As Substrate

dc.creatorde Andrade
dc.creatorCristiano Jose; de Andrade
dc.creatorLidiane Maria; Rocco
dc.creatorSilvana Aparecida; Sforca
dc.creatorMauricio Luis; Pastore
dc.creatorGlaucia Maria; Jauregi
dc.creatorPaula
dc.date2017
dc.datejun
dc.date2017-11-13T13:16:24Z
dc.date2017-11-13T13:16:24Z
dc.date.accessioned2018-03-29T05:53:43Z
dc.date.available2018-03-29T05:53:43Z
dc.identifierSeparation And Purification Technology. Elsevier Science Bv, v. 180, p. 157 - 167, 2017.
dc.identifier1383-5866
dc.identifier1873-3794
dc.identifierWOS:000399516500018
dc.identifier10.1016/j.seppur.2017.02.045
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S1383586617305993
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327539
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1364564
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionP. tsukubaensis is a yeast-like microorganism that synthesized the biosurfactant mannosylerythritol lipids-B (MEL-B). Production cost can be one of the drawbacks of biosurfactants production. Therefore the development of efficient and cost effective purification strategies and the use of by-products in the culture medium could serve as important strategies to reduce overall process cost. The aim of this work was to evaluate the production of MEL using cassava wastewater, a hydrophilic medium composed of a low-cost substrate which is a by-product of cassava processing, followed by foam fractionation and ultra filtration of MEL. Cassava wastewater proved to be a feasible culture medium for P. tsukubaensis and MELB production as the yield (1.26 g L-1) was similar to that reported by others using water-soluble carbon sources (up to 2 g L-1). Interestingly ultrafiltration with 100 kDa MWCO membranes (using 20 mL centrifugal devices) led to the purification of MEL-B in one step since approximate to 80% of MEL was recovered, while more than 95% of proteins were found in the permeate. The scale up of the ultrafiltration (up to 500 mL) using a cross flow filtration unit led to very similar results. Overall the ultrafiltration led to a threefold increase in MEL purity in terms of protein (at both scales). The chemical characterization by NMR confirmed the production of MEL-B homologue and also the production of a second stereoisomer approximate to 9%, while the CG-MS and MALDI-TOFMS analysis confirmed the main fatty acids within the structure of MEL-B (C8:0 and 12:0 and C8:0 and C14:1). Therefore, the process developed here was found to be a good alternative to the conventional production of MEL which uses synthetic culture medium, solvent extraction (ethyl acetate) and column chromatography (silica) for its purification. (C) 2017 Elsevier B.V. All rights reserved.
dc.description180
dc.description157
dc.description167
dc.descriptionSao Paulo State Research Foundation (Fapesp)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherElsevier Science BV
dc.publisherAmsterdam
dc.relationSeparation and Purification Technology
dc.rightsfechado
dc.sourceWOS
dc.subjectPseudozyma Tsukubaensis
dc.subjectCassava Wastewater
dc.subjectMannosylerythritol Lipids-b
dc.subjectUltrafiltration
dc.titleA Novel Approach For The Production And Purification Of Mannosylerythritol Lipids (mel) By Pseudozyma Tsukubaensis Using Cassava Wastewater As Substrate
dc.typeArtículos de revistas


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