dc.creatorRocha
dc.creatorMaria Victoria; Di Giacomo
dc.creatorMelisa; Beltramino
dc.creatorSofia; Loh
dc.creatorWatson; Romanini
dc.creatorDiana; Nerli
dc.creatorBibiana Beatriz
dc.date2016
dc.dateagos
dc.date2017-11-13T13:16:24Z
dc.date2017-11-13T13:16:24Z
dc.date.accessioned2018-03-29T05:53:42Z
dc.date.available2018-03-29T05:53:42Z
dc.identifierSeparation And Purification Technology. Elsevier Science Bv, v. 168, p. 168 - 176, 2016.
dc.identifier1383-5866
dc.identifier1873-3794
dc.identifierWOS:000378951800021
dc.identifier10.1016/j.seppur.2016.05.025
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S1383586616305032?via%3Dihub
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327538
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1364563
dc.descriptionThe role of the natural biodegradable polymer alginate (ALG) as affinity macro-ligand for papain (PAP) was evaluated in order to design a new sustainable two-phase affinity strategy for recovering this enzyme from Carica papaya latex. In presence of PAP, decreased values of intrinsic viscosity and hydrodynamic radius of ALG were observed. These results suggested a neutralizing effect of PAP on the polymer negative charges which caused a decrease of intra-chain repulsion forces in ALG molecules and a consequent shrinkage of the polymer size. Calorimetric measurements demonstrated a cooperative interaction between PAP and ALG which was enthalpy-entropically driven. When partitioned C. papaya latex into aqueous two-phase systems (ATPSs) formed by polyethylene glycol (PEG) of MW 8000 and the biodegradable salt sodium citrate pH 5.20, only 20% of PAP was recovered at the PEG-enriched phase with a purification factor (PF) of 2.48. The addition of ALG 0.1% (w/w) into the system doubled the PAP partition coefficient (K-p,K- PAP), showing the ability of this polymer to enhance the enzyme recovery at the PEG-enriched phase and therefore, increasing the extraction efficiency. The subsequent addition of calcium chloride at a final concentration of 80 mM allowed the precipitation of the target enzyme and the recovering, of PEG phase for recycling. The overall process showed a PAP recovery of 72% and a PF of 2.41. The proposed strategy not only conserved all the advantages of the reported extractions with PEG/ammonium sulfate (or phosphate) ATPSs, i.e., low cost, scalability and integration of the clarification and extraction steps, but also allowed overcoming their main drawbacks, i.e., the separation of the target molecule from the phase-polymer and the environmental impact caused by the disposal of these salts. (C) 2016 Elsevier B.V. All rights reserved.
dc.description168
dc.description168
dc.description176
dc.descriptionCONICET, Argentina [PIP0551/12]
dc.descriptionAgencia Nacional de Promocion Cientifica y Tecnologica, Argentina [FONCyT-PICT-2013-1730]
dc.descriptionFundacion Nuevo Banco de Santa Fe
dc.languageEnglish
dc.publisherElsevier Science BV
dc.publisherAmsterdam
dc.relationSeparation and Purification Technology
dc.rightsfechado
dc.sourceWOS
dc.subjectAffinity Partitioning
dc.subjectAqueous Two-phase Systems
dc.subjectPapain
dc.subjectAlginate
dc.titleA Sustainable Affinity Partitioning Process To Recover Papain From Carica Papaya Latex Using Alginate As Macro-ligand
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución