Separation Of Human Fab Fragments By Negative Chromatography On Omega-aminohexyl- And Poly(ethyleneimine)-agarose

dc.creatorCarmignotto
dc.creatorGabriela Pannunzio; Mourao
dc.creatorCecilia Alves; Alves Bueno
dc.creatorSonia Maria
dc.date2017
dc.datejan
dc.date2017-11-13T13:16:02Z
dc.date2017-11-13T13:16:02Z
dc.date.accessioned2018-03-29T05:53:26Z
dc.date.available2018-03-29T05:53:26Z
dc.identifierProcess Biochemistry. Elsevier Sci Ltd, v. 52, p. 267 - 275, 2017.
dc.identifier1359-5113
dc.identifier1873-3298
dc.identifierWOS:000392774600032
dc.identifier10.1016/j.procbio.2016.10.013
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S135951131630633X
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327475
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1364500
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionPolyamines omega-aminohexyl and poly(ethyleneimine) (PEI) immobilized on agarose gel were evaluated as ligands for the purification of Fab fragments from papain-digested human immunoglobulin G (IgG) solution by negative chromatography. The adsorption of proteolytic fragments was investigated using different buffer systems and pH values. The results showed that the buffer system and pH had stronger effects on the adsorption of the proteolytic fragments onto omega-aminohexyl-agarose than onto PEI-agarose. Tris-HCl buffer pH 8.0 could be used to purify Fab fragments by negative chromatography using both adsorbents, achieving purity and recovery above 96% and 93%, respectively. Static binding experiments were conducted to determine the maximum binding capacity of omega-aminohexyl-agarose adsorbent (Q(m)). The experimental isotherm was best described by the Langmuir-Freundlich model, which indicated the positive cooperativity of adsorption (n = 1.68) and a Q(m) value of 44.32 mg mL(-1) of gel. The results suggest that negative chromatography on omega-aminohexyl- and PEI-agarose is a potential technique for the purification of Fab fragments from papain-digested human IgG solution. (C) 2016 Published by Elsevier Ltd.
dc.description52
dc.description267
dc.description275
dc.descriptionCAPES/PROEX (Brazil)
dc.descriptionCNPq (Brazil)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherElsevier Sci Ltd
dc.publisherOxford
dc.relationProcess Biochemistry
dc.rightsfechado
dc.sourceWOS
dc.subjectHuman Fab Fragments
dc.subjectAminohexyl
dc.subjectPoly(ethyleneimine)
dc.subjectPurification
dc.subjectAdsorption
dc.subjectNegative Chromatography
dc.titleSeparation Of Human Fab Fragments By Negative Chromatography On Omega-aminohexyl- And Poly(ethyleneimine)-agarose
dc.typeArtículos de revistas


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