| dc.creator | Fernandes | |
| dc.creator | Miriam R.; Ignacio | |
| dc.creator | Aline; Rodrigues | |
| dc.creator | Viviane A. A.; Groppo | |
| dc.creator | Franciso C.; Cardoso | |
| dc.creator | Ary L.; Avila-Campos | |
| dc.creator | Mario J.; Nakano | |
| dc.creator | Viviane | |
| dc.date | 2017 | |
| dc.date | jan | |
| dc.date | 2017-11-13T13:14:44Z | |
| dc.date | 2017-11-13T13:14:44Z | |
| dc.date.accessioned | 2018-03-29T05:52:27Z | |
| dc.date.available | 2018-03-29T05:52:27Z | |
| dc.identifier | Microbial Drug Resistance. Mary Ann Liebert Inc, v. 23, p. 56 - +, 2017. | |
| dc.identifier | 1076-6294 | |
| dc.identifier | 1931-8448 | |
| dc.identifier | WOS:000391745500009 | |
| dc.identifier | 10.1089/mdr.2015.0320 | |
| dc.identifier | http://online.liebertpub.com/doi/abs/10.1089/mdr.2015.0320 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/327233 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1364258 | |
| dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
| dc.description | The administration of antimicrobial agents leads to an ecological imbalance of the host-microorganisms relationship, and it causes a rapid and significant reduction in the microbial diversity. The aim of the current study was to evaluate the impact of antibiotic therapy on intestinal microbiota of children between 3 and 12 years of age. The fecal samples were collected from hospitalized children (n = 31) and from healthy untreated children (n = 30). The presence of bacteria and their quantities were assessed by culture-based methods and quantitative polymerase chain reaction (qPCR). By culture method, in the children receiving antibiotics, a low recovery of Bifidobacterium spp. (54.8%), Bacteroides spp./Parabacteroides spp. (54.8%), Clostridium spp. (35.5%), and Escherichia coli (74.2%) was observed compared with the children without antibiotic therapy (100%, 80%, 63.3%, and 86.6%, respectively). By qPCR, the children receiving antibiotics showed a lower copy number for all microorganisms, except to Lactobacillus spp. (p = 0.0092). In comparison to the nontreated children, the antibiotic-treated children showed a significantly lower copy number of Bifidobacterium spp. (p = 0.0002), Clostridium perfringens (p < 0.0001), E. coli (p = 0.0268), Methanobrevibacter smithii (p = 0.0444), and phylum Firmicutes (p = 0.0009). In conclusion, our results obtained through qualitative and quantitative analyses, demonstrate that antibiotic therapy affect the intestinal microbiome of children. | |
| dc.description | 23 | |
| dc.description | 1 | |
| dc.description | 56 | |
| dc.description | + | |
| dc.description | CNPq [158799/2012-7] | |
| dc.description | FAPESP [2013/17739-9] | |
| dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
| dc.language | English | |
| dc.publisher | Mary Ann Liebert Inc | |
| dc.publisher | New Rochelle | |
| dc.relation | Microbial Drug Resistance | |
| dc.rights | fechado | |
| dc.source | WOS | |
| dc.subject | Microbiome | |
| dc.subject | Antibiotic Therapy | |
| dc.subject | Children | |
| dc.subject | Anaerobic Bacteria | |
| dc.subject | Escherichia Coli | |
| dc.title | Alterations Of Intestinal Microbiome By Antibiotic Therapy In Hospitalized Children | |
| dc.type | Artículos de revistas | |
