Tissue Integrity, Costs And Time Associated With Different Agents For Histological Bone Preparation

dc.creatorArtur Abrantes
dc.creatorAdelino Antonio; Rafacho
dc.creatorAlex; Correa Rivero
dc.creatorElena Riet; Mariano
dc.creatorFernanda Viviane; Siqueira
dc.creatorFilipe Modolo; Gondak
dc.creatorRogerio Oliveira
dc.date2017
dc.dateabr
dc.date2017-11-13T13:14:22Z
dc.date2017-11-13T13:14:22Z
dc.date.accessioned2018-03-29T05:52:08Z
dc.date.available2018-03-29T05:52:08Z
dc.identifierMicroscopy Research And Technique. Wiley-blackwell, v. 80, p. 344 - 349, 2017.
dc.identifier1059-910X
dc.identifier1097-0029
dc.identifierWOS:000397853400003
dc.identifier10.1002/jemt.22798
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/jemt.22798/full
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327161
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1364186
dc.descriptionThe selection of an appropriate demineralizing solution in pathology laboratories depends on several factors such as the preservation of cellularity, urgency of diagnostic and financial costs. The aim of this study was to test different decalcification bone procedures in order to establish the best value of these in formalin-fixed and paraffin-embedded samples. Femurs were removed from 13 adult male Wistar rats to obtain 130 bone disks randomly divided into five groups that were demineralized in different concentrationsofnitric acid(GroupI); formic acid (Group II); acetic acid (Group III); EDTA, pH7.4(Group IV) and Morses solution (Group V). Serial, 3-mu m-thick sections were obtained and stained with hematoxylin-eosin to calculate the percentage of osteocyte-occupied lacunae. The sections were also stained with Masson's trichrome in conjunction with picrosirius red under polarized light followed by a semi-quantitative analysis to verify the adjacent muscle-to-bone integrity and preservation of collagen fibres. The highest percentage of osteocyte-occupied lacunae was found with 10% acetic acid solution (95.6460.95%) and Group I (nitric acid) demanded the shorter time (0.8-5.7days). Of all solutions, 5% nitric acid incurred the lowest cost to achieve complete demineralization comparedwith other solutions (p<. 001). Group IV (EDTA) had the highest integrity of muscle and collagen type I and III (P<0.01). Demineralization with 10% acetic acid was the most effective at preserving bone tissue, while 5% EDTA was the best at maintaining collagen and adjacent muscle to bone. In conclusion, nitric acid at 5% showed the most efficient result as it balanced both time and cost as a demineralizing solution.
dc.description80
dc.description4
dc.description344
dc.description349
dc.languageEnglish
dc.publisherWiley-Blackwell
dc.publisherHoboken
dc.relationMicroscopy Research and Technique
dc.rightsfechado
dc.sourceWOS
dc.subjectBone
dc.subjectCost
dc.subjectDemineralization
dc.subjectOsteocytes
dc.subjectOverall Time
dc.titleTissue Integrity, Costs And Time Associated With Different Agents For Histological Bone Preparation
dc.typeArtículos de revistas


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