Artículos de revistas
Sensitive Electroanalytical Detection On Gce: The Case Of Lipoic Acid And Its Interaction With N-acetylcysteine And Glutathione
Registro en:
Electroanalysis. Wiley-v C H Verlag Gmbh, v. 28, p. 2818 - 2826, 2016.
1040-0397
1521-4109
WOS:000387891400025
10.1002/elan.201600205
Autor
Santos
Ana Caroline F.; Moura
Fabiana A.; Tanaka
Auro A.; Luz
Rita C. S.; Damos
Flavio S.; Kubota
Lauro T.; Goulart
Marilia O. F.
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) A simple and sensitive electrochemical method was developed for the determination of N-Acetylcysteine (NAC) and Glutathione (GSH), on glassy carbon electrode. The results indicate that -lipoic acid (LipS(2)) is oxidized at the electrode, but is regenerated by thiols, suggesting a redox catalytic effect. Under optimized conditions, the analytical curves for NAC and GSH were obtained in the range of 1 up to 10 mu M and 1 up to 60 mu M, respectively. The quantification can be performed in a single assay with high sensitivity, reproducibility and low detection limit of 0.27 and 0.67 mu M for NAC and GSH, respectively. This method was successfully applied to the determination of NAC and GSH in artificial saliva and human urine samples, with excellent recovery results. 28 11 2818 2826 CNPq through grants from the CNPq [4840044/2011-3, 407963/2013-8, 458114/2014-6, PRONEX/FAPEAL/CNPq/2009-09-006, PROCAD/CAPES/3004-2014] CAPES through grants from the CNPq [4840044/2011-3, 407963/2013-8, 458114/2014-6, PRONEX/FAPEAL/CNPq/2009-09-006, PROCAD/CAPES/3004-2014] FAPEAL through grants from the CNPq [4840044/2011-3, 407963/2013-8, 458114/2014-6, PRONEX/FAPEAL/CNPq/2009-09-006, PROCAD/CAPES/3004-2014] INCT-Bioanalitica Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)