Breed-specific Factors Influence Embryonic Lipid Composition: Comparison Between Jersey And Holstein

dc.creatorBaldoceda
dc.creatorLuis; Gilbert
dc.creatorIsabelle; Gagne
dc.creatorDominic; Vigneault
dc.creatorChristian; Blondin
dc.creatorPatrick; Ferreira
dc.creatorChristina Ramires; Robert
dc.creatorClaude
dc.date2016
dc.date2017-11-13T13:13:49Z
dc.date2017-11-13T13:13:49Z
dc.date.accessioned2018-03-29T05:51:44Z
dc.date.available2018-03-29T05:51:44Z
dc.identifierReproduction Fertility And Development. Csiro Publishing, v. 28, p. 1185 - 1196, 2016.
dc.identifier1031-3613
dc.identifier1448-5990
dc.identifierWOS:000378930400016
dc.identifier10.1071/RD14211
dc.identifierhttp://www.publish.csiro.au/RD/fulltext/RD14211
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327066
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1364091
dc.descriptionSome embryos exhibit better survival potential to cryopreservation than others. The cause of such a phenotype is still unclear and may be due to cell damage during cryopreservation, resulting from overaccumulation and composition of lipids. In cattle embryos, in vitro culture conditions have been shown to impact the number of lipid droplets within blastomeres. Thus far, the impact of breed on embryonic lipid content has not been studied. In the present study were compared the colour, lipid droplet abundance, lipid composition, mitochondrial activity and gene expression of in vivo-collected Jersey breed embryos, which are known to display poor performance post-freezing, with those of in vivo Holstein embryos, which have good cryotolerance. Even when housed and fed under the same conditions, Jersey embryos were found to be darker and contain more lipid droplets than Holstein embryos, and this was correlated with lower mitochondrial activity. Differential expression of genes associated with lipid metabolism and differences in lipid composition were found. These results show genetic background can impact embryonic lipid metabolism and storage.
dc.description28
dc.description8
dc.description1185
dc.description1196
dc.descriptionNatural Sciences and Engineering Research Council of Canada (NSERC) Strategic Network EmbryoGENE NETPG [340825-06]
dc.languageEnglish
dc.publisherCsiro Publishing
dc.publisherClayton
dc.relationReproduction Fertility and Development
dc.rightsfechado
dc.sourceWOS
dc.subjectEmbryo
dc.subjectLipid Droplets
dc.subjectLipid Profile
dc.subjectMitochondria
dc.titleBreed-specific Factors Influence Embryonic Lipid Composition: Comparison Between Jersey And Holstein
dc.typeArtículos de revistas


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