Antimicrobial Photodynamic Effect Of Extracts And Oxoaporphine Alkaloid Isomoschatoline From Guatteria Blepharophylla

dc.creatorAndreazza
dc.creatorNathalia Luiza; de Lourenco
dc.creatorCaroline Caramano; Hernandez-Tasco
dc.creatorAlvaro Jose; Pinheiro
dc.creatorMaria Lucia B.; Alves Stefanello
dc.creatorMaria Elida; Costa
dc.creatorEmmanoel Vilaca; Salvador
dc.creatorMarcos Jose
dc.date2016
dc.datejul
dc.date2017-11-13T13:13:20Z
dc.date2017-11-13T13:13:20Z
dc.date.accessioned2018-03-29T05:51:20Z
dc.date.available2018-03-29T05:51:20Z
dc.identifierJournal Of Photochemistry And Photobiology And B-biology. Elsevier Science Sa, v. 160, p. 154 - 162, 2016.
dc.identifier1011-1344
dc.identifierWOS:000378453500019
dc.identifier10.1016/j.jphotobiol.2016.04.010
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S1011134416300653?via%3Dihub
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327032
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1364057
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionPhotodynamic Therapy, a tumor therapy idealized at the beginning of the last century, emerges nowadays as a promising treatment alternative against infectious diseases. In this study we report a bioguided study of Guatteria blepharophylla phytoderivatives for antimicrobial PDT. Crude extracts and fraction from the species bark were obtained and further fractionated for substances isolation. All samples were evaluated in relation to their photophysical (absorbance and fluorescence) and photochemical properties (1,3-DPBF bleaching method). Then, bioassays were conducted using as biological models bacteria and yeast strains and a diode laser as a light source. Phytochemical analyses lead to the isolation of 5 isoquinoline alkaloids from oxoaporphine subclass, denominated GB1 to GB5. Photophysical and photochemical analysis showed that extracts, fraction and GB1 (isomoschatoline) presented absorption profile with bands at 600-700 nm and were positive for singlet oxygen production. Photobiological assays indicate that these samples presented photodynamic antimicrobial activity against both gram-positive and gram-negative bacterial and some Candida ssp. yeast strains at sub-inhibitory concentrations. The susceptibility of gram-negative bacteria was significantly enhanced when CaCl2 or MgCl2 were employed. Greater energy doses and double sample's dosage also decreased microbial survival. It is suggested that GB1 photodynamic activity happens through both types I and II photochemical mechanisms, but with a predominance of the latter. Phytoderivatives of G. blepharophylla promoted antimicrobial effect, however more detailed study concerning chemical composition of the crude extracts and fractions as also photophysical and photochemical characteristics of GB1 are necessary to ensure their potential as photosensitizers at antimicrobial photodynamic inactivation. (C) 2016 Elsevier B.V. All rights reserved.
dc.description160
dc.description154
dc.description162
dc.descriptionFundo de Apoio ao Ensino a Pesquisa e Extensao-UNICAMP
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo
dc.descriptionConselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageEnglish
dc.publisherElsevier Science SA
dc.publisherLausanne
dc.relationJournal of Photochemistry and Photobiology and B-Biology
dc.rightsfechado
dc.sourceWOS
dc.subjectAntimicrobial Photodynamic Therapy
dc.subjectAntimicrobial Resistance
dc.subjectGuatteria Blepharophylla
dc.subjectLsoquinoline Alkaloids
dc.subjectOxoaporphine Alkaloid Isomoschatoline
dc.titleAntimicrobial Photodynamic Effect Of Extracts And Oxoaporphine Alkaloid Isomoschatoline From Guatteria Blepharophylla
dc.typeArtículos de revistas


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