Actas de congresos
Mass Spectrometry Screening Of Arabica Coffee Roasting: A Non-target And Non-volatile Approach By Easi-ms And Esi-ms
Registro en:
Food Research International. Elsevier Science Bv, v. 89, p. 967 - 975, 2016.
0963-9969
1873-7145
WOS:000388781400010
10.1016/j.foodres.2016.03.021
Autor
da Rosa
Jeane Santos; Freitas-Silva
Otniel; Costa Rouws
Janaina Ribeiro; da Silva Moreira
Iris Goncalves; Moreira Novaes
Fabio Junior; Azevedo
Debora de Almeida; Schwab
Nicolas; de Oliveira Godoy
Ronoel Luiz; Eberlin
Marcos Nogueira; de Rezende
Claudia Moraes
Institución
Resumen
Coffee roasting needs precise control and innovative techniques that are economically viable to monitor and improve its consistency. In this study, mass spectrometry was used as a tool to screen chemical markers that appear on the surface of coffee beans (whole bean) along the roasting process. A non-target and non-volatile approach was used with an ambient technique (EASI) coupled to a single quadrupole mass analyzer to monitor roasting chemical changes in the coffee bean. Green (raw), soft, medium, dark and very dark roasted coffee beans showed a decrease in ions in the range of m/z 500-600, whereas an increase in abundance in the m/z 800-900 range was clearly observed in the most roasted coffees. A multivariate approach through PCA separated the different roasts in 70% of the variance using PC1 and PC2. The major ions in the range of m/z 500-600 were characterized by ESI-MS and also HPLC-fluorescence as the N-alkanoyltryptamides, surface constituents of coffee wax layer which are almost fully degraded in darker roasts. The ions in the range of m/z 800-900 were characterized as di-and triacylglicerols and its increase during the roasting process was systematically observed. For these classes of chemical markers of the roasting process, ESI-MS showed also the sodium and potassium adducts with good relative abundances. (C) 2016 Elsevier Ltd. All rights reserved. 89 part 2 967 975 3rd International Congress on Cocoa, Coffee and Tea (CoCoTea) JUN 22-24, 2015 Univ Aveiro Campus, Aveiro, PORTUGAL