Identification And Characterization Of Micrornas In The Screwworm Flies Cochliomyia Hominivorax And Cochliomyia Macellaria (diptera: Calliphoridae)

dc.creatorPaulo
dc.creatorD. F.; Azeredo-Espin
dc.creatorA. M. L.; Canesin
dc.creatorL. E. C.; Vicentini
dc.creatorR.; Junqueira
dc.creatorA. C. M.
dc.date2017
dc.datefev
dc.date2017-11-13T13:12:45Z
dc.date2017-11-13T13:12:45Z
dc.date.accessioned2018-03-29T05:50:54Z
dc.date.available2018-03-29T05:50:54Z
dc.identifierInsect Molecular Biology. Wiley-blackwell, v. 26, p. 46 - 57, 2017.
dc.identifier0962-1075
dc.identifier1365-2583
dc.identifierWOS:000391941200005
dc.identifier10.1111/imb.12270
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/imb.12270/abstract
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/326933
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1363958
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionMicroRNAs (miRNAs) are small noncoding RNAs that modulate gene expression through post-transcriptional regulation. Here, we report the identification and characterization of miRNAs in two closely related screw-worm flies with different feeding habits: Cochliomyia hominivorax and Cochliomyia macellaria. The New World screwworm, C. hominivorax, is an obligatory parasite of warm-blooded vertebrates, whereas the secondary screwworm, C. macellaria, is a free-living organism that feeds on decaying organic matter. Here, the small RNA transcriptomes of adults and third-instar larvae of both species were sequenced. A total of 110 evolutionarily conserved miRNAs were identified, and 10 putative precursor miRNAs (pre-miRNAs) were predicted. The relative expression of six selected miRNAs was further investigated, including miRNAs that are related to reproduction and neural processes in other insects. Mature miRNAs were also characterized across an evolutionary time scale, suggesting that the majority of them have been conserved since the emergence of the Arthropoda [540 million years ago (Ma)], Hexapoda (488 Ma) and Brachycera (195 Ma) lineages. This study is the first report of miRNAs for screwworm flies. We also performed a comparative analysis with the hereby predicted miRNAs from the sheep blowfly, Lucilia cuprina. The results presented may advance our understanding of parasitic habits within Calliphoridae and assist further functional studies in blowflies.
dc.description26
dc.description1
dc.description46
dc.description57
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/11654-9]
dc.descriptionFAPESP fellowship [2012/06272-0]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherWiley-Blackwell
dc.publisherHoboken
dc.relationInsect Molecular Biology
dc.rightsfechado
dc.sourceWOS
dc.subjectCochliomyia Hominivorax
dc.subjectCochliomyia Macellaria
dc.subjectScrewworm Fly
dc.subjectBlowfly
dc.subjectMyiasis
dc.subjectParasitism
dc.subjectMicrornas
dc.titleIdentification And Characterization Of Micrornas In The Screwworm Flies Cochliomyia Hominivorax And Cochliomyia Macellaria (diptera: Calliphoridae)
dc.typeArtículos de revistas


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