Hybrid Organic/inorganic Interfaces As Reversible Label-free Platform For Direct Monitoring Of Biochemical Interactions

dc.creatorVello
dc.creatorTatiana P.; da Silva
dc.creatorLarissa M. B.; Silva
dc.creatorGustavo O.; de Camargo
dc.creatorDavi H. S.; Correa
dc.creatorCatia C.; Bof Bufon
dc.creatorCarlos C.
dc.date2017
dc.datejan
dc.date2017-11-13T13:12:11Z
dc.date2017-11-13T13:12:11Z
dc.date.accessioned2018-03-29T05:50:26Z
dc.date.available2018-03-29T05:50:26Z
dc.identifierBiosensors & Bioelectronics. Elsevier Advanced Technology, v. 87, p. 209 - 215, 2017.
dc.identifier0956-5663
dc.identifier1873-4235
dc.identifierWOS:000390499600031
dc.identifier10.1016/j.bios.2016.08.050
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0956566316308053?via%3Dihub
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/326826
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1363851
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionThe combination of organic and inorganic materials to create hybrid nanostructures is an effective approach to develop label-free platforms for biosensing as well as to overcome eventual leakage current related problems in capacitive sensors operating in liquid. In this work, we combine an ultra-thin high-k dielectric layer (Al2O3) with a nanostructured organic functional tail to create a platform capable of monitoring biospecific interactions directly in liquid at very low analyte concentrations. As a proof of concept, a reversible label-free glutathione-S-transferase (GST) biosensor is demonstrated. The sensor can quantify the GST enzyme concentration through its biospecific interaction with tripeptide reduced glutathione (GSH) bioreceptor directly immobilized on the dielectric surface. The enzymatic reaction is monitored by electrical impedance measurements, evaluating variations on the overall capacitance values according to the GST concentration. The biosensor surface can be easily regenerated, allowing the detection of GST with the very same device. The biosensor shows a linear response in the range of 200 mu mol L-1 to 2 mu mol L-1, the largest reported in the literature along with the lowest detectable GST concentration (200 mu mol L-1) for GST label-free sensors. Such a nanostructured hybrid organic-inorganic system represents a powerful tool for the monitoring of biochemical reactions, such as protein-protein interactions, for biosensing and biotechnological applications. (C) 2016 Elsevier B.V. All rights reserved.
dc.description87
dc.description209
dc.description215
dc.descriptionCAPES
dc.descriptionCNPq [483550/2013-2]
dc.descriptionFAPESP [2013/22127-2, 2014/25979-2]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherElsevier Advanced Technology
dc.publisherOxford
dc.relationBiosensors & Bioelectronics
dc.rightsfechado
dc.sourceWOS
dc.subjectHybrid Organic-inorganic Nanostructures
dc.subjectSolid-liquid Interfaces
dc.subjectBiosensor
dc.subjectGlutathione S-transferase
dc.titleHybrid Organic/inorganic Interfaces As Reversible Label-free Platform For Direct Monitoring Of Biochemical Interactions
dc.typeArtículos de revistas


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