Reduced Insulin Secretion Function Is Associated With Pancreatic Islet Redistribution Of Cell Adhesion Molecules (cams) In Diabetic Mice After Prolonged High-fat Diet

dc.creatorFalcao
dc.creatorViviane Tannuri F. L.; Maschio
dc.creatorDaniela A.; de Fontes
dc.creatorCamila Calvo; Oliveira
dc.creatorRicardo B.; Santos-Silva
dc.creatorJunia C.; Soares Almeida
dc.creatorAnna Carolina; Vanzela
dc.creatorEmerielle C.; Cartaxo
dc.creatorMaria Tereza; Carvalho
dc.creatorCarolina P. F.; Collares-Buzato
dc.creatorCarla Beatriz
dc.date2016
dc.datejul
dc.date2017-11-13T13:12:02Z
dc.date2017-11-13T13:12:02Z
dc.date.accessioned2018-03-29T05:50:17Z
dc.date.available2018-03-29T05:50:17Z
dc.identifierHistochemistry And Cell Biology. Springer, v. 146, p. 13 - 31, 2016.
dc.identifier0948-6143
dc.identifier1432-119X
dc.identifierWOS:000378785900002
dc.identifier10.1007/s00418-016-1428-5
dc.identifierhttps://link-springer-com.ez88.periodicos.capes.gov.br/article/10.1007%2Fs00418-016-1428-5
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/326787
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1363812
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionIntercellular junctions play a role in regulating islet cytoarchitecture, insulin biosynthesis and secretion. In this study, we investigated the animal metabolic state as well as islet histology and cellular distribution/expression of CAMs and F-actin in the endocrine pancreas of C57BL/6/JUnib mice fed a high-fat diet (HFd) for a prolonged time period (8 months). Mice fed a HFd became obese and type 2 diabetic, displaying significant peripheral insulin resistance, hyperglycemia and moderate hyperinsulinemia. Isolated islets of HFd-fed mice displayed a significant impairment of glucose-induced insulin secretion associated with a diminished frequency of intracellular calcium oscillations compared with control islets. No marked change in islet morphology and cytoarchitecture was observed; however, HFd-fed mice showed higher beta cell relative area in comparison with controls. As shown by immunohistochemistry, ZO-1, E-, N-cadherins, alpha- and beta-catenins were expressed at the intercellular contact site of endocrine cells, while VE-cadherin, as well as ZO-1, was found at islet vascular compartment. Redistribution of N-, E-cadherins and alpha-catenin (from the contact region to the cytoplasm in endocrine cells) associated with increased submembranous F-actin cell level as well as increased VE-cadherin islet immunolabeling was observed in diabetic mice. Increased gene expression of VE-cadherin and ZO-1, but no change for the other proteins, was observed in islets of diabetic mice. Only in the case of VE-cadherin, a significant increase in islet content of this CAM was detected by immunoblotting in diabetic mice. In conclusion, CAMs are expressed by endocrine and endothelial cells of pancreatic islets. The distribution/expression of N-, E- and VE-cadherins as well as alpha-catenin and F-actin is significantly altered in islet cells of obese and diabetic mice.
dc.description146
dc.description1
dc.description13
dc.description31
dc.descriptionFAPESP [2010/50789-1]
dc.descriptionCNPq
dc.descriptionFAEPEX/UNICAMP
dc.descriptionCAPES/PROEX (Brazil)
dc.descriptionPhD CAPES-DINTER fellowship (Brazil)
dc.descriptionConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil [307163/2012-1, 304991/2015-5]
dc.description[2000/05137-4]
dc.description[2009/54129-9]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherSpringer
dc.publisherNew York
dc.relationHistochemistry and Cell Biology
dc.rightsfechado
dc.sourceWOS
dc.subjectCell Adhesion Molecules
dc.subjectCell-cell Interactions
dc.subjectPancreatic Beta Cells
dc.subjectType 2 Diabetes Mellitus
dc.subjectHigh-fat Diet
dc.subjectInsulin Secretion
dc.titleReduced Insulin Secretion Function Is Associated With Pancreatic Islet Redistribution Of Cell Adhesion Molecules (cams) In Diabetic Mice After Prolonged High-fat Diet
dc.typeArtículos de revistas


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