Immune-inflammatory Cell Profile And Receptor Activator Of Nuclear Factor Kappa B Ligand/osteoprotegerin Expression In Persistent Apical Periodontitis After Root Canal Retreatment Failure

dc.creatorEstrela
dc.creatorCarlos; Decurcio
dc.creatorDaniel de Almeida; Silva
dc.creatorJulio Almeida; Batista
dc.creatorAline Carvalho; de Souza Lima
dc.creatorNathalia Caroline; de Freitas Silva
dc.creatorBrunno Santos; Chaves de Souza
dc.creatorJoao Antonio; Souza Costa
dc.creatorCarlos Alberto
dc.date2016
dc.datemar
dc.date2017-11-13T11:35:15Z
dc.date2017-11-13T11:35:15Z
dc.date.accessioned2018-03-29T05:49:21Z
dc.date.available2018-03-29T05:49:21Z
dc.identifierJournal Of Endodontics. Elsevier Science Inc, v. 42, p. 439 - 446, 2016.
dc.identifier0099-2399
dc.identifier1878-3554
dc.identifierWOS:000372559700015
dc.identifier10.1016/j.joen.2015.11.012
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0099239915010754
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/326581
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1363587
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThis study assessed the immune inflammatory profile and the expression of bone resorption activators receptor activator of nuclear factor kappa B ligand (RANKL) and inhibitor osteoprotegerin (OPG) in apical periodontitis (n = 20) that persisted after root canal retreatment. Methods: Immunohistochemistry was used to characterize lymphocyte populations (CD3(+), CD45R0(+), CD8(+), and FoxP3(+) cells), macrophages (CD68(+)), RANKL(+) and OPG(+) cells in persistent apical periodontitis (PAP) and primary periapical lesions (PPLs). By using quantitative real-time polymerase chain reaction, the mRNA expression of RANKL and OPG in PAP and periodontal ligament from healthy teeth was comparatively analyzed. The data were analyzed by Mann-Whitney, Pearson chi(2), and Wilcoxon tests (5% level). Results: PAP showed an elevated number of FoxP3(+) cells compared with PPL (P < .001). The number of CD68(+) cells was reduced in the PAP samples compared with the PPLs (P < .001). Similar number of other lymphocyte populations was observed in PAP and PPLs (P > .05 for all comparisons). No differences in the RANKL, OPG, and immune-inflammatory cells were demonstrated when comparing PAP microscopically classified as cyst with those classified as granulomas (P > .05 for all comparisons). The assessment of mRNA expression revealed higher levels of RANKL. and OPG in PAP compared with the periodontal ligament from healthy teeth (contiol) samples (P < .001). Also, a greater expression of RANKL in comparison with OPG was observed in PAP (P < .001). Conclusions: These findings indicate that PAP consists of biologically active lesions that demonstrate potential of bone resorption (higher expression of RANKL) and is characterized by an immune-inflammatory cell profile that suggests a suppressive and regulatory environment (higher number of FoxP3(+) cells and lower number of macrophages) favorable to more chronic clinical behavior.
dc.description42
dc.description3
dc.description439
dc.description446
dc.descriptionNational Council for Scientific and Technological Development (CNPq) [474642/2009-7, 306394/2011-1]
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherElsevier Science INC
dc.publisherNew York
dc.relationJournal of Endodontics
dc.rightsfechado
dc.sourceWOS
dc.subjectApical Periodontitis
dc.subjectBone Resorption
dc.subjectImmunological Cells
dc.subjectOsteoprotegerin
dc.subjectRank Ligand
dc.titleImmune-inflammatory Cell Profile And Receptor Activator Of Nuclear Factor Kappa B Ligand/osteoprotegerin Expression In Persistent Apical Periodontitis After Root Canal Retreatment Failure
dc.typeArtículos de revistas


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