Artículos de revistas
Co-distribution Of Cysteine Cathepsins And Matrix Metalloproteases In Human Dentin
Registro en:
Archives Of Oral Biology. Pergamon-elsevier Science Ltd , v. 74, p. 101 - 107, 2017.
0003-9969
1879-1506
WOS:000393017400015
10.1016/j.archoralbio.2016.11.011
Autor
Candia Scaffa
Polliana Mendes; Breschi
Lorenzo; Mazzoni
Annalisa; Pimenta Vidal
Cristina de Mattos; Curci
Rosa; Apolonio
Fabianni; Gobbi
Pietro; Pashley
David; Tjaderhane
Leo; dos Santos Tersariol
Ivarne Luis; Nascimento
Fabio Dupart; Carrilhon
Marcela Rocha
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) It has been hypothesized that cysteine cathepsin's (CTs) along with matrix metalloproteases (MMPs) may work in conjunction in the proteolysis of mature dentin matrix. The aim of this study was to verify simultaneously the distribution and presence of cathepsins B (CT-B) and K (CT-K) in partially demineralized dentin; and further to evaluate the activity of CTs and MMPs in the same tissue. The distribution of CT-B and CT-K in sound human dentin was assessed by immunohistochemistry. A double-immunolabeling technique was used to identify, at once, the occurrence of those enzymes in dentin. Activities of CTs and MMPs in dentin extracts were evaluated spectrofluorometrically. In addition, in situ gelatinolytic activity of dentin was assayed by zymography. The results revealed the distribution of CT-B and CT-K along the dentin organic matrix and also indicated co-occurrence of MMPs and CTs in that tissue. The enzyme kinetics studies showed proteolytic activity in dentin extracts for both classes of proteases. Furthermore, it was observed that, at least for sound human dentin matrices, the activity of MMPs seems to be predominant over the CTs one. (C) 2016 Elsevier Ltd. All rights reserved. 74 101 107 FAPESP, Brazil [2007/54618-4, 2009/14005-9] CNPq, Brazil [312768/2013-3] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)