Neuropilin Controls Endothelial Differentiation By Mesenchymal Stem Cells From The Periodontal Ligament

dc.creatorAmorim
dc.creatorBruna R.; Silverio
dc.creatorKarina G.; Casati
dc.creatorMarcio Z.; Sallum
dc.creatorEnilson A.; Kantovitz
dc.creatorKamila R.; Nociti
dc.creatorFrancisco H.
dc.creatorJr.
dc.date2016
dc.datejul
dc.date2017-11-13T11:32:53Z
dc.date2017-11-13T11:32:53Z
dc.date.accessioned2018-03-29T05:47:25Z
dc.date.available2018-03-29T05:47:25Z
dc.identifierJournal Of Periodontology. Amer Acad Periodontology, v. 87, p. E138 - E147, 2016.
dc.identifier0022-3492
dc.identifier1943-3670
dc.identifierWOS:000379254600002
dc.identifier10.1902/jop.2016.150603
dc.identifierhttp://www-joponline-org.ez88.periodicos.capes.gov.br/doi/abs/10.1902/jop.2016.150603
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/326153
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1363159
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionPeriodontal ligament (PDL) has been reported to be a source of mesenchymal stem cells (MSCs). New vascular networks from undifferentiated cells are essential for repair/regeneration of specialized tissues, including PDL. The current study aims to determine potential of CD105(+)-enriched cell subsets of periodontal ligament cells (PDLSCs) to differentiate into endothelial cell (EC)-like cells and to give insights into the mechanism involved. Methods: CD105(+)-enriched PDLSCs were induced to EC differentiation by endothelial growth medium 2 (EGM-2) for 3, 7, 14, and 21 days, with mRNA/protein levels and functional activity assessed by: 1) real-time polymerase chain reaction; 2) Western blotting; 3) fluorescence-activated cell sorting; 4) immunohistochemistry; 5) immunofluorescence; 6) matrigel; and 7) small interfering RNA assays. Results: Data analyses demonstrated that EGM-2 treated PDLSCs presented increased expression of EC markers, including: 1) CD105; 2) kinase domain-containing receptor; and 3) Ulex europaeus agglutinin 1, and were able to form cord/tube-like structures. Gene and protein expression analysis showed that neuropilin 2 (NRP2), a key factor for vascular development, was significantly downregulated during EC differentiation. NRP2 was constitutively expressed in mouse PDL tissues by immunohistochemistry analysis, and NRP2 knockdown in CD105(+)-enriched PDLSCs resulted in increased cord/tube-like structures in a matrigel assay. Conclusion: These findings demonstrated the potential of CD105(+)-enriched PDLSCs to support angiogenesis, and NRP2 as a pivotal factor regulating this process.
dc.description87
dc.description7
dc.descriptionE138
dc.descriptionE147
dc.descriptionCoordination for Improvement of Higher Education Personnel, Postdoctoral National Program (CAPES-PNPD, Minister of Education, Brazil) [02426/09-9]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageEnglish
dc.publisherAmer Acad Periodontology
dc.publisherChicago
dc.relationJournal of Periodontology
dc.rightsfechado
dc.sourceWOS
dc.subjectCell Differentiation
dc.subjectEndothelial Cells
dc.subjectNeuropilin-2
dc.subjectPeriodontal Ligament
dc.subjectPrimary Cell Culture
dc.subjectStem Cells
dc.titleNeuropilin Controls Endothelial Differentiation By Mesenchymal Stem Cells From The Periodontal Ligament
dc.typeArtículos de revistas


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