Artículos de revistas
Characterization Of Crude And Partially Purified Lipase From Geotrichum Candidum Obtained With Different Nitrogen Sources
Registro en:
Journal Of The American Oil Chemists Society. Springer, v. 93, p. 1355 - 1364, 2016.
0003-021X
1558-9331
WOS:000384424800004
10.1007/s11746-016-2875-9
Autor
Maldonado
Rafael Resende; Pozza
Eduardo Luiz; Aguiar-Oliveira
Elizama; Almeida Costa
Fatima Aparecida; Maugeri Filho
Francisco; Rodrigues
Maria Isabel
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Lipases from Geotrichum candidum were produced in two different medium: A = 12 % (w/v) clarified corn steep liquor (CCSL) + 0.6 % (w/v) soybean oil (SO) and B = 3.5 % (w/v) yeast hydrolysate (YH) + 0.7 % (w/v) SO. Lipases were partially purified from both media by hydrophobic interaction chromatography using 3.0 mol L-1 of NaCl as mobile phase, and they were characterized in the crude and partially purified forms. The recovery of lipase activity from CCSL and YH via HIC were 96 and 94.3 %, and the purification factors were 44.3 and 86.7-fold, respectively. All evaluated lipases had similar optimum pH (7.0-7.7), but, for the CCSL crude lipase, optimum temperature (47 A degrees C) was 10 A degrees C higher than others lipases evaluated. CCSL crude lipase possessed a higher thermo stability than YH crude lipase, e.g., at 37 A degrees C (pH 7.0) the half-life of CCSL crude lipase was 19.25 h and at pH 8.0 (30 A degrees C) the half-life was 48 h, which are five and ten times higher than with YH crude lipase, respectively. On the other hand, the YH crude lipase possessed a higher catalytic constant (k (cat) = 2.3 min(-1)) but with almost the same catalytic efficiency (K (m)/k (cat) = 32.12 mg mL min(-1)) in relation to CCSL crude lipase. The lipases differ in biocatalytic properties between substrates, suggesting that the two lipases can be employed for different applications. 93 10 1355 1364 Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Brazil) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil) Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)