Esi-ms Fingerprinting Of Residues Of Green Propolis, And Evaluation Of Their Antioxidant And Antimicrobial Activities

dc.creatorCorrea
dc.creatorWallace Ribeiro; Lopez
dc.creatorBegona Gimenez-Cassina; do Prado
dc.creatorSheila Cristina; da Silva Cunha
dc.creatorIldenize Barbosa; Helena Frankland Sawaya
dc.creatorAlexandra Christine; Salvador
dc.creatorMarcos Jose
dc.date2016
dc.date2017-11-13T11:32:06Z
dc.date2017-11-13T11:32:06Z
dc.date.accessioned2018-03-29T05:46:50Z
dc.date.available2018-03-29T05:46:50Z
dc.identifierJournal Of Apicultural Research. Taylor & Francis Ltd, v. 55, p. 1 - 7, 2016.
dc.identifier0021-8839
dc.identifier2078-6913
dc.identifierWOS:000382966500001
dc.identifier10.1080/00218839.2016.1196014
dc.identifierhttp://www.tandfonline.com/doi/abs/10.1080/00218839.2016.1196014
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/326020
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1363026
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionIn this study, we evaluated the composition of the residues of subsequent extractions of raw propolis compared to that of the first extraction (EEP) in order to determine the appropriate number of extractions that could be used. Three samples of green propolis, two of them from small apiaries and another from a larger apiary (all with similar composition) were analyzed. The chemical composition of the residues and the crude propolis was evaluated by direct insertion electrospray ionization mass spectrometry in the negative ion mode (ESI(-)-MS) using a TQD Acquity mass spectrometer. The antioxidant activity was assessed by DPPH and ORAC assays, and the antimicrobial activity was studied as well. A comparison of the ESI(-)-MS fingerprints showed that bioactive substances still remain in the residues after one or two extractions. Artepillin C was found to be present in green propolis extract (EEP) and also in the residues; however it was present in lower concentrations. Nevertheless, another important substance, dicaffeoylquinic acid, was only found in the propolis extract and in the first two residues. In the DPPH and ORAC assays, a sequential loss of antioxidant activity was observed. The antimicrobial activity of EEP and extract of the first two residues was investigated in vitro against gram-positive and gram-negative bacteria and EEP, and the first extract presented inhibition values of 1mgml(-1) or less. Two sequential extractions of raw green propolis are appropriate to avoid the loss of important chemical components in the residue.
dc.description55
dc.description1
dc.description1
dc.description7
dc.descriptionCAPES
dc.descriptionFAPESP [2013/3020313-3, 2008/58035-6]
dc.descriptionIFSULDEMINAS
dc.descriptionFAEPEX
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherTaylor & Francis Ltd
dc.publisherAbingdon
dc.relationJournal of Apicultural Research
dc.rightsfechado
dc.sourceWOS
dc.subjectElectrospray Ionization Mass Spectrometry Fingerprinting
dc.subjectPropolis
dc.subjectAntioxidant Activity
dc.subjectAntimicrobial Activity
dc.titleEsi-ms Fingerprinting Of Residues Of Green Propolis, And Evaluation Of Their Antioxidant And Antimicrobial Activities
dc.typeArtículos de revistas


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