Microrna And Protein Profiles In Invasive Versus Non-invasive Oral Tongue Squamous Cell Carcinoma Cells In Vitro

dc.creatorKorvala
dc.creatorJohanna; Jee
dc.creatorKowan; Porkola
dc.creatorEmmi; Almangush
dc.creatorAlhadi; Mosakhani
dc.creatorNeda; Bitu
dc.creatorCarolina; Cervigne
dc.creatorNilva K.; Zandonadi
dc.creatorFlavia S.; Meirelles
dc.creatorGabriela V.; Paes Leme
dc.creatorAdriana Franco; Coletta
dc.creatorRicardo D.; Leivo
dc.creatorIlmo; Salo
dc.creatorTuula
dc.date2017
dc.datejan
dc.date2017-11-13T11:31:34Z
dc.date2017-11-13T11:31:34Z
dc.date.accessioned2018-03-29T05:46:27Z
dc.date.available2018-03-29T05:46:27Z
dc.identifierExperimental Cell Research. Elsevier Inc, v. 350, p. 9 - 18, 2017.
dc.identifier0014-4827
dc.identifier1090-2422
dc.identifierWOS:000392366800002
dc.identifier10.1016/j.yexcr.2016.10.015
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0014482716303408
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/325932
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1362938
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionComplex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated "Focal adhesion" and "ECM-receptor interaction" as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren't significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.
dc.description350
dc.description1
dc.description9
dc.description18
dc.descriptionFinnish Cancer Society
dc.descriptionSigrid Juselius Foundation
dc.descriptionOulu University Hospital MRC
dc.descriptionVTR funding
dc.descriptionFinska Lakaresallskapet foundation
dc.descriptionMaritza and Reino Salonen Foundation
dc.descriptionTurku University Hospital VTR funding
dc.descriptionFinnish Cultural Foundation
dc.descriptionThelma Makikyro Foundation
dc.descriptionScience without borders (CAPES, Brazil)
dc.descriptionFAPESP [2009/54067-3, 2010/19278-0]
dc.descriptionCNPq [470268/2013-1]
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPES, Brasilia, Brazil [AUXPE-PVES-570/2013]
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP, Sao Paulo, Brazil [2013/01607-6]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageEnglish
dc.publisherElsevier INC
dc.publisherSan Diego
dc.relationExperimental Cell Research
dc.rightsfechado
dc.sourceWOS
dc.subjectMicrorna
dc.subjectProteomics
dc.subjectOral Tongue Squamous Cell Carcinoma
dc.subjectBioinformatics
dc.titleMicrorna And Protein Profiles In Invasive Versus Non-invasive Oral Tongue Squamous Cell Carcinoma Cells In Vitro
dc.typeArtículos de revistas


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