Integrated Analysis Of Recombinant Bpv-1 L1 Protein For The Production Of A Bovine Papillomavirus Vlp Vaccine

dc.creatorMódolo D.G.
dc.creatorAraldi R.P.
dc.creatorMazzuchelli-de-Souza J.
dc.creatorPereira A.
dc.creatorPimenta D.C.
dc.creatorZanphorlin L.M.
dc.creatorBeçak W.
dc.creatorMenossi M.
dc.creatorde Cassia Stocco R.
dc.creatorde Carvalho R.F.
dc.date2017
dc.date2017-08-17T19:17:54Z
dc.date2017-08-17T19:17:54Z
dc.date.accessioned2018-03-29T05:27:38Z
dc.date.available2018-03-29T05:27:38Z
dc.identifierVaccine. Elsevier Ltd, v. 35, n. 12, p. 1590 - 1593, 2017.
dc.identifier0264-410X
dc.identifier10.1016/j.vaccine.2017.02.007
dc.identifierhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85013127894&doi=10.1016%2fj.vaccine.2017.02.007&partnerID=40&md5=7566e0e879b625f8819fb271a2a8af38
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/324213
dc.identifier2-s2.0-85013127894
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1358376
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionBovine papillomatosis is an infectious disease that is caused by bovine papillomavirus (BPV), which results in important economic losses. However, no BPV vaccines or effective treatment methods are commercially available to date. Moreover, the absence of papillomavirus replication in vitro makes the use of recombinant protein a promising candidate for vaccine formulations. Hence, we developed an integrated study on the L1 capsid protein of BPV-1, obtained from a bacterial expression system, regarding its purification, biosafety, thermostability and immunogenicity. The results indicated an absence of genotoxicity of the purified recombinant L1 protein, β-sheet prevalence of secondary structure folding, protein stability under high temperatures as well as the presence of capsomeres and VLPs. In addition, preliminary experimental vaccination of calves showed the production of specific antibodies against BPV-1 L1. © 2017 Elsevier Ltd
dc.description35
dc.description12
dc.description1590
dc.description1593
dc.descriptionCNPq, Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherElsevier Ltd
dc.relationVaccine
dc.rightsfechado
dc.sourceScopus
dc.subjectBiosafety
dc.subjectBovine Papillomavirus
dc.subjectCapsomeres
dc.subjectL1 Protein
dc.subjectVaccine
dc.subjectVlp
dc.titleIntegrated Analysis Of Recombinant Bpv-1 L1 Protein For The Production Of A Bovine Papillomavirus Vlp Vaccine
dc.typeArtículos de revistas


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