Carcinoma Ex-pleomorphic Adenoma Derived From Recurrent Pleomorphic Adenoma Shows Important Difference By Array Cgh Compared To Recurrent Pleomorphic Adenoma Without Malignant Transformation

Carcinoma Ex-adenoma Pleomórfico Derivado De Adenoma Pleomórfico Recorrente Mostra Diferença Importante Por Array Cgh Em Comparação Com Adenoma Pleomórfico Recorrente Sem Transformação Maligna

dc.creatorMariano F.V.
dc.creatorGiovanetti K.
dc.creatorSaccomani L.F.V.
dc.creatorDel Negro A.
dc.creatorKowalski L.P.
dc.creatorKrepischi A.C.V.
dc.creatorAltemani A.
dc.date2016
dc.date2017-08-17T19:17:42Z
dc.date2017-08-17T19:17:42Z
dc.date.accessioned2018-03-29T05:27:18Z
dc.date.available2018-03-29T05:27:18Z
dc.identifierBrazilian Journal Of Otorhinolaryngology. Elsevier Editora Ltda, v. 82, n. 6, p. 687 - 694, 2016.
dc.identifier1808-8694
dc.identifier10.1016/j.bjorl.2015.12.004
dc.identifierhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84959921789&doi=10.1016%2fj.bjorl.2015.12.004&partnerID=40&md5=585925d2d7d346065eabec22972979c7
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/324137
dc.identifier2-s2.0-84959921789
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1358300
dc.descriptionA key step of cancer development is the progressive accumulation of genomic changes resulting in disruption of several biological mechanisms. Carcinoma ex-pleomorphic adenoma (CXPA) is an aggressive neoplasm that arises from a pleomorphic adenoma. CXPA derived from a recurrent PA (RPA) has been rarely reported, and the genomic changes associated with these tumors have not yet been studied. Objective We analyzed CXPA from RPAs and RPAs without malignant transformation using array-comparative genomic hybridization (array-CGH) to identify somatic copy number alterations and affected genes. Methods DNA samples extracted from FFPE tumors were submitted to array-CGH investigation, and data was analyzed by Nexus Copy Number Discovery Edition v.7. Results No somatic copy number alterations were found in RPAs without malignant transformation. As for CXPA from RPA, although genomic profiles were unique for each case, we detected some chromosomal regions that appear to be preferentially affected by copy number alterations. The first case of CXPA-RPA (frankly invasive myoepithelial carcinoma) showed copy number alterations affecting 1p36.33p13, 5p and chromosomes 3 and 8. The second case of CXPA-RPA (frankly invasive epithelial-myoepithelial carcinoma) showed several alterations at chromosomes 3, 8, and 16, with two amplifications at 8p12p11.21 and 12q14.3q21.2. The third case of CXPA-RPA (minimally invasive epithelial-myoepithelial carcinoma) exhibited amplifications at 12q13.3q14.1, 12q14.3, and 12q15. Conclusion The occurrence of gains at chromosomes 3 and 8 and genomic amplifications at 8p and 12q, mainly those encompassing the HMGA2, MDM2, WIF1, WHSC1L1, LIRG3, CDK4 in CXAP from RPA can be a significant promotional factor in malignant transformation. © 2016 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial
dc.description82
dc.description6
dc.description687
dc.description694
dc.languageEnglish; Portuguese
dc.publisherElsevier Editora Ltda
dc.relationBrazilian Journal of Otorhinolaryngology
dc.rightsaberto
dc.sourceScopus
dc.subjectAcgh
dc.subjectCarcinoma Ex-pleomorphic Adenoma
dc.subjectRecurrent Pleomorphic Adenoma
dc.subjectSomatic Copy Number Alterations
dc.titleCarcinoma Ex-pleomorphic Adenoma Derived From Recurrent Pleomorphic Adenoma Shows Important Difference By Array Cgh Compared To Recurrent Pleomorphic Adenoma Without Malignant Transformation
dc.titleCarcinoma Ex-adenoma Pleomórfico Derivado De Adenoma Pleomórfico Recorrente Mostra Diferença Importante Por Array Cgh Em Comparação Com Adenoma Pleomórfico Recorrente Sem Transformação Maligna
dc.typeArtículos de revistas


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