This study aimed to build and to gather evidence of validity for a scale of teaching, learning and motivational strategies for learning in online learning environments (EEAM-AVA). To investigate evidence for the validity of the scale, the internal structure and dimensions that emerged during this study were considered. Participating in the survey were 572 students enrolled on courses in mediated virtual learning environments. The research instrument was built using a scale of forty five items. The analysis of the data collected showed a structure of five dimensions, all with acceptable levels of inner consistency, indicating the validity of the scale and its psychometric properties. Additional studies to revise and improve the items presented on the scale were recommended. It is hoped that the results may be of benefit for educators and psychologists who research questions investigated in this study.2166593611