Rna Sequencing Reveals Region-specific Molecular Mechanisms Associated With Epileptogenesis In A Model Of Classical Hippocampal Sclerosis

dc.creatorVieira
dc.creatorAS; de Matos
dc.creatorAH; do Canto
dc.creatorAM; Rocha
dc.creatorCS; Carvalho
dc.creatorBS; Pascoal
dc.creatorVDB; Norwood
dc.creatorB; Bauer
dc.creatorS; Rosenow
dc.creatorF; Gilioli
dc.creatorR; Cendes
dc.creatorF; Lopes-Cendes
dc.creatorI
dc.date2016
dc.date2016-12-06T18:32:24Z
dc.date2016-12-06T18:32:24Z
dc.date.accessioned2018-03-29T02:04:59Z
dc.date.available2018-03-29T02:04:59Z
dc.identifier
dc.identifierScientific Reports. NATURE PUBLISHING GROUP, n. 6, n. 22416, p. .
dc.identifier2045-2322
dc.identifierWOS:000371226400001
dc.identifier10.1038/srep22416
dc.identifierhttp://www-nature-com.ez88.periodicos.capes.gov.br/articles/srep22416
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/320530
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1311296
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionWe report here the first complete transcriptome analysis of the dorsal (dDG) and ventral dentate gyrus (vDG) of a rat epilepsy model presenting a hippocampal lesion with a strict resemblance to classical hippocampal sclerosis (HS). We collected the dDG and vDG by laser microdissection 15 days after electrical stimulation and performed high-throughput RNA-sequencing. There were many differentially regulated genes, some of which were specific to either of the two sub-regions in stimulated animals. Gene ontology analysis indicated an enrichment of inflammation-related processes in both subregions and of axonal guidance and calcium signaling processes exclusively in the vDG. There was also a differential regulation of genes encoding molecules involved in synaptic function, neural electrical activity and neuropeptides in stimulated rats. The data presented here suggests, in the time point analyzed, a remarkable interaction among several molecular components which takes place in the damaged hippocampi. Furthermore, even though similar mechanisms may function in different regions of the DG, the molecular components involved seem to be region specific.
dc.description6
dc.description
dc.description
dc.description
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), SP, BRAZIL
dc.descriptionFAPESP
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), BRAZIL
dc.descriptionConselho Nacional de Pesquisa (CNPq), Brazil
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description
dc.description
dc.description
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.publisherLONDON
dc.relationScientific Reports
dc.rightsaberto
dc.sourceWOS
dc.subjectTemporal-lobe Epilepsy
dc.subjectInduced Status Epilepticus
dc.subjectPilocarpine Model
dc.subjectRat Hippocampus
dc.subjectNeuropeptide-y
dc.subjectClinicopathological Classification
dc.subjectGaba(a) Receptor
dc.subjectDown-regulation
dc.subjectAwake Rats
dc.subjectAdult-rats
dc.titleRna Sequencing Reveals Region-specific Molecular Mechanisms Associated With Epileptogenesis In A Model Of Classical Hippocampal Sclerosis
dc.typeArtículos de revistas


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