Evidence Of Allopolyploidy In Urochloa Humidicola Based On Cytological Analysis And Genetic Linkage Mapping

dc.creatorVigna
dc.creatorBBZ; Santos
dc.creatorJCS; Jungmann
dc.creatorL; do Valle
dc.creatorCB; Mollinari
dc.creatorM; Pastina
dc.creatorMM; Pagliarini
dc.creatorMS; Garcia
dc.creatorAAF; Souza
dc.creatorAP
dc.date2016
dc.date2016-12-06T18:32:13Z
dc.date2016-12-06T18:32:13Z
dc.date.accessioned2018-03-29T02:04:48Z
dc.date.available2018-03-29T02:04:48Z
dc.identifier
dc.identifierPlos One. PUBLIC LIBRARY SCIENCE, n. 11, n. 4, p. .
dc.identifier1932-6203
dc.identifierWOS:000374565100021
dc.identifier10.1371/journal.pone.0153764
dc.identifierhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0153764
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/320482
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1311248
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe African species Urochloa humidicola (Rendle) Morrone & Zuloaga (syn. Brachiaria humidicola (Rendle) Schweick.) is an important perennial forage grass found throughout the tropics. This species is polyploid, ranging from tetra to nonaploid, and apomictic, which makes genetic studies challenging; therefore, the number of currently available genetic resources is limited. The genomic architecture and evolution of U. humidicola and the molecular markers linked to apomixis were investigated in a full-sib F-1 population obtained by crossing the sexual accession H031 and the apomictic cultivar U. humidicola cv. BRS Tupi, both of which are hexaploid. A simple sequence repeat (SSR)-based linkage map was constructed for the species from 102 polymorphic and specific SSR markers based on simplex and double-simplex markers. The map consisted of 49 linkage groups (LGs) and had a total length of 1702.82 cM, with 89 microsatellite loci and an average map density of 10.6 cM. Eight homology groups (HGs) were formed, comprising 22 LGs, and the other LGs remained ungrouped. The locus that controls apospory (apo-locus) was mapped in LG02 and was located 19.4 cM from the locus Bh027.c.D2. In the cytological analyses of some hybrids, bi-to hexavalents at diakinesis were observed, as well as two nucleoli in some meiocytes, smaller chromosomes with preferential allocation within the first metaphase plate and asynchronous chromosome migration to the poles during anaphase. The linkage map and the meiocyte analyses confirm previous reports of hybridization and suggest an allopolyploid origin of the hexaploid U. humidicola. This is the first linkage map of an Urochloa species, and it will be useful for future quantitative trait locus (QTL) analysis after saturation of the map and for genome assembly and evolutionary studies in Urochloa spp. Moreover, the results of the apomixis mapping are consistent with previous reports and confirm the need for additional studies to search for a co-segregating marker.
dc.description11
dc.description
dc.description
dc.description
dc.descriptionBrazilian Agricultural Research Corporation (Embrapa)
dc.descriptionBrazilian National Council for Scientific and Technological Development (CNPq) [478262/2004-3, 502336/2005-6, 482458/2007-0]
dc.descriptionState of Sao Paulo Research Foundation (FAPESP) [2005/51010-0, 2008/52197-4]
dc.descriptionFoundation for Science and Technological Development of the State of Mato Grosso do Sul (Fundect)
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.descriptionFAPESP [2007/57022-5, 2010/50032-8]
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES - EMBRAPA Program)
dc.descriptionCNPq [304914/2010-0]
dc.descriptionCNPq
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description
dc.description
dc.description
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.publisherSAN FRANCISCO
dc.relationPlos One
dc.rightsaberto
dc.sourceWOS
dc.subjectPoaceae Panicoideae Paniceae
dc.subjectDose Restriction Fragments
dc.subjectSpp. Commercial Cross
dc.subjectGenomic Region
dc.subjectPennisetum-squamulatum
dc.subjectBrachiaria-brizantha
dc.subjectCenchrus-ciliaris
dc.subjectMeiotic Behavior
dc.subjectSaccharum Spp.
dc.subjectAflp Markers
dc.titleEvidence Of Allopolyploidy In Urochloa Humidicola Based On Cytological Analysis And Genetic Linkage Mapping
dc.typeArtículos de revistas


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