Separation Of Human Igg Fragments Using Copper, Nickel, Zinc, And Cobalt Chelated To Cm-asp-agarose By Positive And Negative Chromatography

dc.creatorMourao
dc.creatorCA; Carmignotto
dc.creatorGP; Bueno
dc.creatorSMA
dc.date2016
dc.date2016-12-06T18:31:46Z
dc.date2016-12-06T18:31:46Z
dc.date.accessioned2018-03-29T02:04:23Z
dc.date.available2018-03-29T02:04:23Z
dc.identifier1873-376X
dc.identifierJournal Of Chromatography B. ELSEVIER SCIENCE BV, n. 1017-1018, p. 163 - 173.
dc.identifier1570-0232
dc.identifierWOS:000373547200020
dc.identifier10.1016/j.jchromb.2016.01.058
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S1570023216300587
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/320375
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1311141
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionThis study evaluated the feasibility of using immobilized metal-ion affinity chromatography (IMAC) for separation of human Fab fragments using four different transition metal ions copper, nickel, zinc, and cobalt chelated to CM-Asp (carboxymethylaspartate) immobilized on the agarose gel. The Fab and Fc fragments (from human IgG digested with papain) interacted differently with the chelates studied, depending on the adsorption buffer system. The interaction between chelate and Fc fragment is predominantly based on the coordination bonds using adsorption buffer containing NaCl. Negative chromatography was performed on Cu(II)-CM-Asp-agarose obtaining 2.9 mg of Fab per mL of adsorbent in nonretained fractions (Fc fragment-free without uncleaved IgG). The adsorption of Fab fragments is governed by electrostatic forces in the absence of NaCl in the adsorption buffer. High selectivity was achieved on Co(II)-CM-Asp-agarose and 5.7 mg of Fab per mL of adsorbent was obtained in eluted fractions without Fc fragments, although having uncleaved IgG. The results showed that chromatography on transition metal ions chetated to CM-Asp-agarose is a promising approach to separation of Fab fragments from papain-digested human IgG solution. (C) 2016 Elsevier B.V. All rights reserved.
dc.description1017-1018
dc.description
dc.description163
dc.description173
dc.descriptionCNPq
dc.descriptionCAPES
dc.descriptionCAPES/PROEX, Brazil
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description
dc.description
dc.description
dc.languageEnglish
dc.publisherELSEVIER SCIENCE BV
dc.publisherAMSTERDAM
dc.relationJournal of Chromatography B
dc.rightsfechado
dc.sourceWOS
dc.subjectHuman Fab Fragments
dc.subjectImac
dc.subjectCm-asp (carboxymethylaspartate)
dc.subjectAdsorption
dc.subjectNegative Chromatography
dc.titleSeparation Of Human Igg Fragments Using Copper, Nickel, Zinc, And Cobalt Chelated To Cm-asp-agarose By Positive And Negative Chromatography
dc.typeArtículos de revistas


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