Insights Into The Plant Polysaccharide Degradation Potential Of The Xylanolytic Yeast Pseudozyma Brasiliensis

dc.creatorNeto
dc.creatorAAK; Borin
dc.creatorGP; Goldman
dc.creatorGH; Damasio
dc.creatorARD; Oliveira
dc.creatorJVD
dc.date2016
dc.date2016-12-06T18:31:45Z
dc.date2016-12-06T18:31:45Z
dc.date.accessioned2018-03-29T02:04:21Z
dc.date.available2018-03-29T02:04:21Z
dc.identifier1567-1364
dc.identifierFems Yeast Research. OXFORD UNIV PRESS, n. 117, n. 2, p. .
dc.identifier1567-1356
dc.identifierWOS:000373103000005
dc.identifier10.1093/femsyr/fov117
dc.identifierhttp://femsyr.oxfordjournals.org.ez88.periodicos.capes.gov.br/content/16/2/fov117
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/320370
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1311136
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionIn second-generation (2G) bioethanol production, plant cell-wall polysaccharides are broken down to release fermentable sugars. The enzymes of this process are classified as carbohydrate-active enzymes (CAZymes) and contribute substantially to the cost of biofuel production. A novel basidiomycete yeast species, Pseudozyma brasiliensis, was recently discovered. It produces an endo-beta-1,4-xylanase with a higher specific activity than other xylanases. This enzyme is essential for the hydrolysis of biomass-derived xylan and has an important role in 2G bioethanol production. In spite of the P. brasiliensis biotechnological potential, there is no information about how it breaks down polysaccharides. For the first time, we characterized the secretome of P. brasiliensis grown on different carbon sources (xylose, xylan, cellobiose and glucose) and also under starvation conditions. The growth and consumption of each carbohydrate and the activity of the CAZymes of culture supernatants were analyzed. The CAZymes found in its secretomes, validated by enzymatic assays, have the potential to hydrolyze xylan, mannan, cellobiose and other polysaccharides. The data show that this yeast is a potential source of hydrolases, which can be used for biomass saccharification.
dc.description117
dc.description
dc.description
dc.description
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP 2014/11766-7, 2015/08222-8, 2013/18910-3]
dc.descriptionCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description
dc.description
dc.description
dc.languageEnglish
dc.publisherOXFORD UNIV PRESS
dc.publisherOXFORD
dc.relationFEMS Yeast Research
dc.rightsfechado
dc.sourceWOS
dc.subjectPseudozyma Brasiliensis
dc.subjectBasidiomycete
dc.subjectCazyme
dc.subjectSecretome
dc.subjectSecond-generation Bioethanol
dc.subjectXylanolytic Yeast
dc.titleInsights Into The Plant Polysaccharide Degradation Potential Of The Xylanolytic Yeast Pseudozyma Brasiliensis
dc.typeArtículos de revistas


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