The Anti-biofilm Potential Of Commonly Discarded Agro-industrial Residues Against Opportunistic Pathogens

dc.creatorRochelle
dc.creatorSLD; Sardi
dc.creatorJDO; Freires
dc.creatorIA; Galvao
dc.creatorLCD; Lazarini
dc.creatorJG; de Alencar
dc.creatorSM; Rosalen
dc.creatorPL
dc.date2016
dc.date2016-12-06T18:30:42Z
dc.date2016-12-06T18:30:42Z
dc.date.accessioned2018-03-29T02:03:17Z
dc.date.available2018-03-29T02:03:17Z
dc.identifier1872-633X
dc.identifierIndustrial Crops And Products. ELSEVIER SCIENCE BV, n. 87, p. 150 - 160.
dc.identifier0926-6690
dc.identifierWOS:000377323100019
dc.identifier10.1016/j.indcrop.2016.03.044
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S092666901630190X
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/320099
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1310865
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionBrazil is one of the largest producers of agro-industrial residues in the world. The aim of the study was to evaluate the anti-biofilm potential of hydroalcoholic extracts from agro-industrial residues. Organic residues from different parts were obtained from companies and subjected to hydroalcoholic extraction. A total of 14 extracts from the species Theobroma cacao (cocoa), Coffea arabica (coffee), Psidium guajava (guava), Citrus sinensis (orange), Malus domestica (apple), geopropolis, Punica granatum (pomegranate) and Solanum lycopersicum (tomato) were obtained and screened for their MIC/MBC against reference microorganisms (eleven bacterial strains and one yeast strain). The most bioactive extracts were chemically characterized by GC/MS and tested for their ability to inhibit adhesion, kill mature biofilm cells, and disrupt biofilm morphology. Eight extracts showed antimicrobial activity with MIC values ranging from 31.25 to 2000 mu g/ml and MBC/MFC ranging from 62.50 to 4000 mu g/ml. The extracts of geopropolis, pomegranate batchs #1 and #2 and coffee were able to inhibit adhesion at their MICs by 23% to 80% depending on the strain. At 10 x MIC, three extracts also inhibited mature biofilm of seven pathogens. Toxicity tests were performed in vivo with the extracts of geopropolis, pomegranate I and pomegranate 2 in G. mellonella larvae. At the dose of 50 mg/kg, the extracts did not exert considerable acute toxic effects in the larvae over a period of 72 h. The extracts from agro-industrial residues have promising anti-biofilm activity against opportunistic pathogens of clinical relevance in the medical and dental settings. (C) 2016 Published by Elsevier B.V.
dc.description87
dc.description
dc.description150
dc.description160
dc.descriptionNational Council for Scientific and Technological Development (CNPq, Brazil) [140029/2014-1, 308644/2011-5]
dc.descriptionCoordination for the Improvement of Higher Education Personnel (CAPES-Proex)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description
dc.description
dc.description
dc.languageEnglish
dc.publisherELSEVIER SCIENCE BV
dc.publisherAMSTERDAM
dc.relationIndustrial Crops and Products
dc.rightsfechado
dc.sourceWOS
dc.subjectAgro-industrial Residues
dc.subjectAdhesion
dc.subjectBiofilm
dc.subjectOpportunistic Pathogens
dc.titleThe Anti-biofilm Potential Of Commonly Discarded Agro-industrial Residues Against Opportunistic Pathogens
dc.typeArtículos de revistas


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