[des-arg(1)]-proctolin: A Novel Nep-like Enzyme Inhibitor Identified In Tityus Serrulatus Venom

dc.creatorDuzzi
dc.creatorB; Cajado-Caryalho
dc.creatorD; Kuniyoshi
dc.creatorAK; Kodama
dc.creatorRT; Gozzo
dc.creatorFC; Fioramonte
dc.creatorM; Tambourgi
dc.creatorDV; Portaro
dc.creatorFV; Rioli
dc.creatorV
dc.date2016
dc.date2016-12-06T18:29:59Z
dc.date2016-12-06T18:29:59Z
dc.date.accessioned2018-03-29T02:02:33Z
dc.date.available2018-03-29T02:02:33Z
dc.identifier1873-5169
dc.identifierPeptides. ELSEVIER SCIENCE INC, n. 80, p. 18 - 24.
dc.identifier1873-5169
dc.identifierWOS:000375936400004
dc.identifier10.1016/j.peptides.2015.05.013
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0196978115001692
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/319925
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1310691
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe scorpion Tityus serrulatus venom comprises a complex mixture of molecules that paralyzes and kills preys, especially insects. However, venom components also interact with molecules in humans, causing clinic envenomation. This cross-interaction may result from homologous molecular targets in mammalians and insects, such as (NEP)-like enzymes. In face of these similarities, we searched for peptides in Tityus serrulatus venom using human NEP as a screening tool. We found a NEP-inhibiting peptide with the primary sequence YLPT, which is very similar to that of the insect neuropeptide proctolin (RYLPT). Thus, we named the new peptide [des-Arg(1)]-proctolin. Comparative NEP activity assays using natural substrates demonstrated that [des-Arg(1)]-proctolin has high specificity for NEP and better inhibitory activity than proctolin. To test the initial hypothesis that molecular homologies allow Tityus serrulatus venom to act on both mammal and insect targets, we investigated the presence of a NEP-like in cockroaches, the main scorpion prey, that could be likewise inhibited by [des-Arg(1)]-proctolin. Indeed, we detected a possible NEP-like in a homogenate of cockroach heads whose activity was blocked by thiorphan and also by [des-Arg(1)]-proctolin. Western blot analysis using a human NEP monoclonal antibody suggested a NEP-like enzyme in the homogenate of cockroach heads. Our study describes for the first time a proctolin-like peptide, named [des-Arg(1)]-proctolin, isolated from Tityus serrulatus venom. The tetrapeptide inhibits human NEP activity and a NEP-like activity in a cockroach head homogenate, thus it may play a role in human envenomation as well as in the paralysis and death of scorpion preys. (C) 2015 Elsevier Inc. All rights reserved.
dc.description80
dc.description
dc.description18
dc.description24
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo [2014/12976-5, 2012/06677-0, 2013/15343-0]
dc.descriptionInstituto Nacional de Ciencia e Tecnologia em Toxinas-Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.descriptionConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description
dc.description
dc.description
dc.languageEnglish
dc.publisherELSEVIER SCIENCE INC
dc.publisherNEW YORK
dc.relationPeptides
dc.rightsfechado
dc.sourceWOS
dc.subjectTityus Serrulatus
dc.subjectNeprilysin (nep)
dc.subjectProctolin
dc.subject[des-arg(1)]-proctolin
dc.subjectCockroach
dc.subjectScorpion Venom
dc.subjectNeuropeptide
dc.title[des-arg(1)]-proctolin: A Novel Nep-like Enzyme Inhibitor Identified In Tityus Serrulatus Venom
dc.typeArtículos de revistas


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