Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices

dc.creatorDellagnezze
dc.creatorBruna Martins; Vasconcellos
dc.creatorSuzan Pantaroto de; Melo
dc.creatorItamar Soares de; Santos Neto
dc.creatorEugênio Vaz dos; Oliveira
dc.creatorValéria Maia de
dc.date2016
dc.date2016-11-10T19:22:50Z
dc.date2016-11-10T19:22:50Z
dc.date.accessioned2018-03-29T01:59:21Z
dc.date.available2018-03-29T01:59:21Z
dc.identifierBrazilian Journal Of Microbiology, 47, 3, p.712-. 2016.
dc.identifier1678-4405
dc.identifierS1517-83822016000300712
dc.identifier10.1016/j.bjm.2016.04.004
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300712
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/318963
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1309845
dc.descriptionUnraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene libraries in order to compare the microbial biomass yield, DNA recovery efficiency and diversity among the enrichments. The DNA from microbial communities in petroleum enrichments was purified according to a protocol established in this work and used for 16S rRNA amplification with bacterial generic primers. The PCR products were cloned, and positive clones were screened by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sequencing and phylogenetic analyses revealed that the bacterial community was mostly represented by members of the genera Petrotoga, Bacillus, Pseudomonas, Geobacillus and Rahnella. The use of different support materials in the enrichments yielded an increase in microbial biomass and biofilm formation, indicating that these materials may be employed for efficient biomass recovery from petroleum reservoir samples. Nonetheless, the most diverse microbiota were recovered from the biodegraded petroleum sample using polyurethane foam cubes as support material.
dc.description
dc.description47
dc.description3
dc.description712
dc.description723
dc.languageEnglish
dc.relationBrazilian Journal of Microbiology
dc.relationBrazilian Journal of Microbiology
dc.rightsaberto
dc.sourceSciELO
dc.titleEvaluation of bacterial diversity recovered from petroleum samples using different physical matrices
dc.title
dc.typeArtículos de revistas


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