Use of the second harmonic generation microscopy to evaluate chondrogenic differentiation of mesenchymal stem cells for cartilage repair

dc.creatorBordeaux-Rego, P.
dc.creatorBaratti, M. O.
dc.creatorDuarte, A. S. S.
dc.creatorRibeiro, T. B.
dc.creatorAndreoli-Risso, M. F.
dc.creatorVidal, B.
dc.creatorMiranda, J. B.
dc.creatorAdur, J.
dc.creatorde Thomaz, A. A.
dc.creatorPelegati, V. B.
dc.creatorCosta, F. F.
dc.creatorCarvalho, H. F.
dc.creatorCesar, C. L.
dc.creatorLuzo, A.
dc.creatorOlalla Saad, S. T.
dc.date2012
dc.date2013-09-19T18:06:23Z
dc.date2016-07-01T15:40:44Z
dc.date2013-09-19T18:06:23Z
dc.date2016-07-01T15:40:44Z
dc.date.accessioned2018-03-29T01:56:06Z
dc.date.available2018-03-29T01:56:06Z
dc.identifierMultiphoton Microscopy In The Biomedical Sciences Xii. Spie-Int Soc Optical Engineering, v.8226, 2012
dc.identifier0277-786X
dc.identifierWOS:000302556900054
dc.identifier10.1117/12.909185
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/2118
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/2118
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1308959
dc.descriptionArticular cartilage injury remains one of the major concerns in orthopedic surgery. Mesenchymal stem cell (MSC) transplantation has been introduced to avoid some of the side effects and complications of current techniques.. With the aim to evaluate chondrogenic differentiation of mesenchymal stem cells, we used Second Harmonic Generation (SHG) microscopy to analyze the aggregation and orientation of collagen fibrils in the hyaline cartilage of rabbit knees. The experiment was performed using implants with type II collagen hydrogel (a biomaterial that mimics the microenvironment of the cartilage), one implant containing MSC and one other without MSC (control). After 10 weeks, the rabbit knees were dissected and fibril collagen distribution and spatial organization in the extracellular matrix of the lesions were verified by SHG. The result showed significant differences, whereas in histological sections of the cartilaginous lesions with MSC the collagen fibers are organized and regular; in the control sections the collagen fibers are more irregular, with absence of cells. A macroscopic analysis of the lesions confirmed this difference, showing a greater percentage of lesions filling in knees treated with MSC than in the knees used as controls. This study demonstrates that SHG microscopy will be an excellent tool to help in the evaluation of the effectiveness of MSC-based cell therapy for cartilage repair.
dc.descriptionConference on Multiphoton Microscopy in the Biomedical Sciences XII
dc.description8226
dc.languageeng
dc.publisherSpie-Int Soc Optical Engineering
dc.publisherBellingham
dc.relationMultiphoton Microscopy In The Biomedical Sciences Xii
dc.rightsfechado
dc.sourceWOS
dc.subjectSecond Harmonic Generation
dc.subjectmesenchymal stem cell
dc.subjectcartilage repair collagen fibrils
dc.subjectBONE-MARROW
dc.subjectARTICULAR-CARTILAGE
dc.subjectIN-VITRO
dc.subjectMATRIX
dc.subjectTISSUE
dc.subjectCHONDROCYTES
dc.subjectEXPRESSION
dc.subjectSITU
dc.titleUse of the second harmonic generation microscopy to evaluate chondrogenic differentiation of mesenchymal stem cells for cartilage repair
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución