Neuronal degeneration and gliosis time-course in the mouse hippocampal formation after pilocarpine-induced status epilepticus

dc.creatordo Nascimento, Andre Luiz
dc.creatordos Santos, Neide Ferreira
dc.creatorPelagio, Fernanda Campos
dc.creatorTeixeira, Simone Aparecida
dc.creatorDe Moraes Ferrari, Elenice A.
dc.creatorLangone, Francesco
dc.date2012
dc.date2013-09-19T18:06:50Z
dc.date2016-07-01T15:12:20Z
dc.date2013-09-19T18:06:50Z
dc.date2016-07-01T15:12:20Z
dc.date.accessioned2018-03-29T01:55:28Z
dc.date.available2018-03-29T01:55:28Z
dc.identifierBrain Research. Elsevier, v.1470, p.98-110, 2012
dc.identifier0006-8993
dc.identifierWOS:000308679500012
dc.identifier10.1016/j.brainres.2012.06.008
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/2446
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/2446
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1308804
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionTemporal lobe epilepsy (TLE) is the most common type of human epilepsy and has been related with extensive loss of hippocampal pyramidal and dentate hilar neurons and gliosis. Many characteristics of TLE are reproduced in the pilocarpine model of epilepsy in mice. This study analyzed the neuronal damage, assessed with Fluoro-Jade (FJB) and cresyl violet, and gliosis, investigated with glial fibrilary acidic protein (GFAP) immunohistochemistry, occurring in the hippocampal formation of mice at 3, 6, 12 and 24 h, 1 and 3 weeks after the pilocarpine-induced status-epilepticus (SE) onset. The maximum neuronal damage score and the FJB-positive neurons peak were found in the hilus of dentate gyrus 3 and 12h after SE onset (P<0.05), respectively. At 1 week after SE onset, the greatest neuronal damage score was detected in the CA1 pyramidal cell layer and the greatest numbers of FJB-positive neurons were found both in the CA1 and CA3 pyramidal cell layers (P<0.05). The molecular, CA3 and CA1 pyramidal cell layers expressed highest presence of GFAP immunoreaction at 1 and 3 weeks after SE onset (P<0.05). Our findings show that, depending on the affected area, neuronal death and gliosis can occur within few hours or weeks after SE onset. Our results corroborate previous studies and characterize short time points of temporal evolution of neuropathological changes after the onset of pilocarpine-induced SE in mice and evidences that additional studies of this temporal evolution may be useful to the comprehension of the cellular mechanisms underlying epileptogenesis. (C) 2012 Elsevier B.V. All rights reserved.
dc.description1470
dc.description98
dc.description110
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherElsevier
dc.publisherAmsterdam
dc.relationBrain Research
dc.rightsfechado
dc.sourceWOS
dc.subjectEpilepsy
dc.subjectStatus epilepticus
dc.subjectPilocarpine
dc.subjectFluoro-jade B
dc.subjectNeurodegeneration
dc.subjectAstrocyte
dc.subjectTEMPORAL-LOBE EPILEPSY
dc.subjectTONIC-CLONIC SEIZURES
dc.subjectDENTATE GYRUS
dc.subjectKAINIC ACID
dc.subjectMESSENGER-RNA
dc.subjectMODEL
dc.subjectRATS
dc.subjectEXPRESSION
dc.subjectDEATH
dc.subjectMICE
dc.titleNeuronal degeneration and gliosis time-course in the mouse hippocampal formation after pilocarpine-induced status epilepticus
dc.typeArtículos de revistas


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