Molecular cloning and insecticidal effect of Inga laurina trypsin inhibitor on Diatraea saccharalis and Heliothis virescens

dc.creatorRamos, Vanessa da S.
dc.creatorCabrera, Odalys G.
dc.creatorCamargo, Eduardo L. O.
dc.creatorAmbrosio, Alinne B.
dc.creatorVidal, Ramon O.
dc.creatorda Silva, Desiree S.
dc.creatorGuimaraes, Lays C.
dc.creatorMarangoni, Sergio
dc.creatorParra, Jose R. P.
dc.creatorPereira, Goncalo A. G.
dc.creatorMacedo, Maria L. R.
dc.date2012
dc.date2013-09-19T18:06:34Z
dc.date2016-07-01T14:41:57Z
dc.date2013-09-19T18:06:34Z
dc.date2016-07-01T14:41:57Z
dc.date.accessioned2018-03-29T01:54:38Z
dc.date.available2018-03-29T01:54:38Z
dc.identifierComparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology. Elsevier, v.156, n.41367, p.148-158, 2012
dc.identifier1532-0456
dc.identifierWOS:000309434200003
dc.identifier10.1016/j.cbpc.2012.07.007
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/2266
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/2266
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1308594
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionNative Inga laurina (Fabaceae) trypsin inhibitor (ILTI) was tested for anti-insect activity against Diatraea saccharalis and Heliothis virescens larvae. The addition of 0.1% ILTI to the diet of D. saccharalis did not alter larval survival but decreased larval weight by 51%. The H. virescens larvae that were fed a diet containing 0.5% ILTI showed an 84% decrease in weight. ILTI was not digested by the midgut proteinases of either species of larvae. The trypsin levels were reduced by 55.3% in the feces of D. saccharalis and increased by 24.1% in the feces of H. virescens. The trypsin activity in both species fed with ILTI was sensitive to the inhibitor, suggesting that no novel proteinase resistant to ILTI was induced. Additionally, ILTI exhibited inhibitory activity against the proteinases present in the larval midgut of different species of Lepidoptera. The organization of the ilti gene was elucidated by analyzing its corresponding genomic sequence. The recombinant ILTI protein (reILTI) was expressed and purified, and its efficacy was evaluated. Both native ILTI and reILTI exhibited a similar strong inhibitory effect on bovine trypsin activity. These results suggest that ILTI presents insecticidal properties against both insects and may thus be a useful tool in the genetic engineering of plants. (c) 2012 Elsevier Inc. All rights reserved.
dc.description156
dc.description03/abr
dc.description148
dc.description158
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFUNDECT (Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado do Mato Grosso do Sul)
dc.descriptionPROPP/UFMS (Pro-Reitoria de Pesquisa e Pos-graduacao da Universidade Federal de Mato Grosso do Sul)
dc.descriptionFINEP (Financiamento de Estudos e Projetos/Ministerio da Ciencia e Tecnologia)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherElsevier
dc.publisherNew York
dc.relationComparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology
dc.rightsfechado
dc.sourceWOS
dc.subjectProteinase trypsin inhibitor
dc.subjectBiological control
dc.subjectCloning
dc.subjectPlant-insect interaction
dc.subjectSOYBEAN PROTEINASE-INHIBITOR
dc.subjectSINGLE DISULFIDE BRIDGE
dc.subjectPROTEASE INHIBITORS
dc.subjectKUNITZ-TYPE
dc.subjectTRANSGENIC PLANTS
dc.subjectCALLOSOBRUCHUS-MACULATUS
dc.subjectDIGESTIVE PROTEINASES
dc.subjectHELICOVERPA-ARMIGERA
dc.subjectANAGASTA-KUEHNIELLA
dc.subjectSPODOPTERA-LITURA
dc.titleMolecular cloning and insecticidal effect of Inga laurina trypsin inhibitor on Diatraea saccharalis and Heliothis virescens
dc.typeArtículos de revistas


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