dc.creatorNOVAES, Leticia C. de Lencastre
dc.creatorMAZZOLA, Priscila G.
dc.creatorPESSOA JR., Adalberto
dc.creatorPENNA, Thereza C. Vessoni
dc.date2010
dc.date2013-07-26T17:57:53Z
dc.date2016-07-01T12:55:53Z
dc.date2013-07-26T17:57:53Z
dc.date2016-07-01T12:55:53Z
dc.date.accessioned2018-03-29T01:53:54Z
dc.date.available2018-03-29T01:53:54Z
dc.identifierBIOTECHNOLOGY PROGRESS, v.26, n.1, p.252-256, 2010
dc.identifier8756-7938
dc.identifier10.1002/btpr.296
dc.identifierhttp://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000274973400032&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
dc.identifierhttp://dx.doi.org/10.1002/btpr.296
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/486
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/486
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1308514
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionGreen fluorescent protein (GFP) shows remarkable structural stability and high fluorescence; its stability can be directly related to its fluorescence output, among other characteristics. GFP is stable under increasing temperatures, and its thermal denaturation is highly reproducible. Some polymers, such as polyethylene glycol, are often used as modifiers of characteristics of biological macromolecules, to improve the biochemical activity and stability of proteins or drug bioavailability. The aim of this study was to evaluate the thermal stability of GFP in the presence of different PEG molar weights at several concentrations and exposed to constant temperatures, in a range of 70-95 degrees C. Thermal stability was expressed in decimal reduction time. It was observed that the D-values obtained were almost constant for temperatures of 85, 90, and 95 degrees C, despite the PEG concentration or molar weight studied. Even though PEG can stabilize proteins, only at 75 degrees C, PEG 600 and 4,000 g/mol stabilized GFP. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 26: 252-256, 2010
dc.description26
dc.description1
dc.description252
dc.description256
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherJOHN WILEY & SONS LTD
dc.publisherInglaterra
dc.relationBiotechnology Progress
dc.rightsfechado
dc.rightsCopyright JOHN WILEY & SONS LTD
dc.sourceWOS
dc.subjectgreen fluorescent protein
dc.subjectpolyethylene glycol
dc.subjectprotein stability
dc.subjectthermal stability
dc.subjectESCHERICHIA-COLI
dc.subjectGFP
dc.subjectSYSTEM
dc.subjectPH
dc.subjectBiotechnology & Applied Microbiology
dc.subjectFood Science & Technology
dc.titleEffect of Polyethylene Glycol on the Thermal Stability of Green Fluorescent Protein
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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