Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis

dc.creatorCASTRO, Leandro M.
dc.creatorBERTI, Denise A.
dc.creatorRUSSO, Lilian C.
dc.creatorCOELHO, Veronica
dc.creatorGOZZO, Fabio C.
dc.creatorOLIVEIRA, Vitor
dc.creatorFERRO, Emer S.
dc.date2010
dc.date2013-07-26T17:58:11Z
dc.date2016-07-01T12:54:07Z
dc.date2013-07-26T17:58:11Z
dc.date2016-07-01T12:54:07Z
dc.date.accessioned2018-03-29T01:53:44Z
dc.date.available2018-03-29T01:53:44Z
dc.identifierAAPS JOURNAL, v.12, n.4, p.608-616, 2010
dc.identifier1550-7416
dc.identifier10.1208/s12248-010-9224-y
dc.identifierhttp://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000288426100014&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
dc.identifierhttp://dx.doi.org/10.1208/s12248-010-9224-y
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/623
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/623
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1308470
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCells produce and use peptides in distinctive ways. In the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role.
dc.description12
dc.description4
dc.description608
dc.description616
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFinanciadora de Estudos e Projetos (FINEP)[A-03/134]
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionInstituto de Investigacao em Imunologia iii-Instituto Nacional de Ciencia e Tecnologia (INCT)[573879/2008-7]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherSPRINGER
dc.publisherEstados Unidos
dc.relationAaps Journal
dc.rightsfechado
dc.rightsCopyright SPRINGER
dc.sourceWOS
dc.subjectintracellular peptide
dc.subjectmass spectrometry
dc.subjectMHC-I antigen presentation
dc.subjectpeptidome
dc.subjectproteasome
dc.subjectMHC CLASS-I
dc.subjectCD8+ T-CELLS
dc.subjectTHIMET OLIGOPEPTIDASE
dc.subjectANTIGEN PRESENTATION
dc.subjectABC TRANSPORTERS
dc.subjectMUTANT MICE
dc.subjectSUBSTRATE-SPECIFICITY
dc.subjectPROTEIN INTERACTIONS
dc.subjectENDOPEPTIDASE 24.15
dc.subjectNATURAL REGULATORS
dc.subjectPharmacology & Pharmacy
dc.titleSimilar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución