Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development

dc.creatorMoraes, Angela M.
dc.creatorJorge, Soraia A. C.
dc.creatorAstray, Renato M.
dc.creatorSuazo, Claudio A. T.
dc.creatorCalderon Riquelme, Camilo E.
dc.creatorAugusto, Elisabeth F. P.
dc.creatorTonso, Aldo
dc.creatorPamboukian, Marilena M.
dc.creatorPiccoli, Rosane A. M.
dc.creatorBarral, Manuel F.
dc.creatorPereira, Carlos A.
dc.date2012
dc.date2013-09-19T18:06:56Z
dc.date2016-06-30T19:52:05Z
dc.date2013-09-19T18:06:56Z
dc.date2016-06-30T19:52:05Z
dc.date.accessioned2018-03-29T01:53:19Z
dc.date.available2018-03-29T01:53:19Z
dc.identifierBiotechnology Advances. Pergamon-Elsevier Science Ltd, v.30, n.3, p.613-628, 2012
dc.identifier0734-9750
dc.identifierWOS:000301901500011
dc.identifier10.1016/j.biotechadv.2011.10.009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/2521
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/2521
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1308370
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionIn the present review we discuss strategies that have been used for heterologous gene expression in Drosophila melanogaster Schneider 2 (S2) cells using plasmid vectors. Since the growth of S2 cells is not dependent on anchorage to solid substrates, these cells can be easily cultured in suspension in large volumes. The factors that most affect the growth and gene expression of S2 cells, namely cell line, cell passage, inoculum concentration, culture medium, temperature, dissolved oxygen concentration, pH, hydrodynamic forces and toxic metabolites, are discussed by comparison with other insect and mammalian cells. Gene expression, cell metabolism, culture medium formulation and parameters involved in cellular respiration are particularly emphasized. The experience of the authors with the successful expression of a biologically functional protein, the rabies virus glycoprotein (RVGP), by recombinant S2 cells is presented in the topics covered. (C) 2011 Elsevier Inc. All rights reserved.
dc.description30
dc.description3
dc.description613
dc.description628
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionMillipore Industria e Comercio Ltda.
dc.descriptionInterprise Instrumentos Analiticos Ltda.
dc.descriptionCultilab Materiais para Cultura de Celulas Ltda.
dc.descriptionAmbriex S/A
dc.descriptionGE Healthcare do Brasil Ltda.
dc.descriptionInvitrogen Brasil Ltda.
dc.descriptionBiosystems, BD, Interlab Distribuidora de Produtos Cientificos Ltda.
dc.descriptionVallee S.A.
dc.descriptionFundacao Butantan
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherPergamon-Elsevier Science Ltd
dc.publisherOxford
dc.relationBiotechnology Advances
dc.rightsfechado
dc.sourceWOS
dc.subjectDrosophila melanogaster
dc.subjectSchneider S2 cells
dc.subjectGene expression
dc.subjectHeterologous genes
dc.subjectRecombinant proteins
dc.subjectRabies virus glycoprotein
dc.subjectBioprocess
dc.subjectSCHNEIDER 2 CELLS
dc.subjectRABIES-VIRUS GLYCOPROTEIN
dc.subjectRECOMBINANT PROTEIN-PRODUCTION
dc.subjectGREEN FLUORESCENT PROTEIN
dc.subjectSPODOPTERA-FRUGIPERDA SF9
dc.subjectOXYGEN-UPTAKE RATE
dc.subjectSERUM-FREE MEDIUM
dc.subjectSECRETED HUMAN ERYTHROPOIETIN
dc.subjectBETA-N-ACETYLGLUCOSAMINIDASE
dc.subjectLONOMIA-OBLIQUA HEMOLYMPH
dc.titleDrosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development
dc.typeArtículos de revistas


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