Effect of combined treatment of hydrolysis and polymerization with transglutaminase on beta-lactoglobulin antigenicity

dc.creatorSabadin, Isabele Serimarco
dc.creatorVillas-Boas, Mariana Battaglin
dc.creatorZollner, Ricardo de Lima
dc.creatorNetto, Flavia Maria
dc.date2012
dc.date2013-09-19T18:06:36Z
dc.date2016-06-30T18:49:35Z
dc.date2013-09-19T18:06:36Z
dc.date2016-06-30T18:49:35Z
dc.date.accessioned2018-03-29T01:53:02Z
dc.date.available2018-03-29T01:53:02Z
dc.identifierEuropean Food Research and Technology. Springer, v.235, n.5, p.801-809, 2012
dc.identifier1438-2377
dc.identifierWOS:000309869500005
dc.identifier10.1007/s00217-012-1802-z
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/2298
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/2298
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1308304
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionThe effect of combined treatments of hydrolysis with different proteases, and subsequent polymerization with transglutaminase on the antigenic activity of beta-Lg was studied. For the hydrolysis of beta-Lg using Alcalase, Neutrase or bromelain, the reaction conditions were 3 % beta-Lg and enzyme:substrate 25 U g(-1) of protein, as was defined using factorial study. Under these conditions, the degree of hydrolysis (DH) of the hydrolysates was 12.6 % when obtained with Alcalase and approximately 4 % with Neutrase or bromelain. Post-hydrolysis polymerization did not result in an increase in molecular mass of the protein, but these samples presented a lower DH, determined by trinitrobenzenosulfonic acid (TNBS) method, suggesting that polymerization had occurred. Hydrolysis with the three enzymes reduced the beta-Lg antigenicity, as evaluated by ELISA and immunoblotting analyses. The IgE-binding responses were practically null (< 9 mu g mL(-1)), 22.82 and 55.73 mu g mL(-1) towards the hydrolysates obtained with Alcalase, bromelain, and Neutrase, respectively. The post-hydrolysis polymerization increased or had no significant effect (P a parts per thousand yen 0.05) on the antigenic response of the hydrolysates.
dc.description235
dc.description5
dc.description801
dc.description809
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherSpringer
dc.publisherNew York
dc.relationEuropean Food Research and Technology
dc.rightsfechado
dc.sourceWOS
dc.subjectHydrolysis
dc.subjectCrosslink
dc.subjectTransglutaminase
dc.subjectAntigenicity
dc.subjectBovine milk proteins
dc.subjectENZYMATIC CROSS-LINKING
dc.subjectCOWS MILK ALLERGY
dc.subjectWHEY PROTEINS
dc.subjectMICROBIAL TRANSGLUTAMINASE
dc.subjectHIGH-PRESSURE
dc.subjectALPHA-LACTALBUMIN
dc.subjectCELL EPITOPES
dc.subjectIGE-BINDING
dc.subjectALLERGENICITY
dc.subjectACID
dc.titleEffect of combined treatment of hydrolysis and polymerization with transglutaminase on beta-lactoglobulin antigenicity
dc.typeArtículos de revistas


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